Distribution of the putative virulence factor encoding gene sheta in Staphylococcus hyicus strains of various origins.

Distribution of the putative virulence factor encoding gene sheta in Staphylococcus hyicus strains of various origins.
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DOI:
10.4142/jvs.2008.9.3.327
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发表时间:
2008-09
影响因子:
1.8
通讯作者:
Skvortzov VN
Skvortzov VN
中科院分区:
农林科学4区
文献类型:
--
作者:
Kanbar T;Voytenko AV;Alber J;Lämmler C;Weiss R;Skvortzov VN

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在本研究中,葡萄球菌(S.)研究了在俄罗斯(n = 23)和德国(n = 17)分离的猪属菌株中先前描述的基因sheta和shetb的流行。16株S. hyicus菌株。Sheta阳性菌株主要见于渗出性表皮炎分离的菌株中,并且经常与剥脱毒素编码基因exhD和exhC一起存在。单个S. hyicus菌株的sheta序列与GenBank中的sheta序列几乎完全匹配。没有一个S。hyicus菌株与本研究中使用的shetb特异性寡核苷酸引物显示阳性反应。根据目前的研究结果,外毒素编码基因sheta可能分布在S。俄罗斯和德国的Hyicus菌株。这种外毒素不具有葡萄球菌剥脱毒素的经典结构,其致炎潜力仍有待阐明。
In the present study, Staphylococcus (S.) hyicus strains isolated in Russia (n = 23) and Germany (n = 17) were investigated for the prevalence of the previously described genes sheta and shetb. Sheta was detected in 16 S. hyicus strains. Sheta-positive strains were mainly found among strains isolated from exudative epidermitis, and frequently together with the exfoliative toxin-encoding genes exhD and exhC. Partial sequencing of sheta in a single S. hyicus strain revealed an almost complete match with the sheta sequence obtained from GenBank. None of the S. hyicus strains displayed a positive reaction with the shetb-specific oligonucleotide primer used in the present study. According to the present results, the exotoxin encoding gene sheta seems to be distributed among S. hyicus strains in Russia and Germany. The toxigenic potential of this exotoxin, which does not have the classical structure of a staphylococcal exfoliative toxin, remains to be elucidated.
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