The gender-specific impact of starvation on mitotypes diversity in adults of Drosophila melanogaster.
The gender-specific impact of starvation on mitotypes diversity in adults of Drosophila melanogaster.
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在动物中，饥饿可以增加某些组织中的活性氧（ROS）水平。线粒体DNA （mtDNA）由于缺乏组蛋白保护，更容易受到ROS的攻击，导致氧化损伤。然而，饥饿是否与mtDNA的遗传多样性有关仍不清楚。本文以黑腹果蝇成年个体为研究对象，在三种不同的摄食处理下（饥饿、只提供水和正常摄食），基于线粒体基因细胞色素c氧化酶亚基I （mt-cox1）部分序列PCR扩增子的高通量测序结果，三个处理组线粒体单倍型的平均数量和个体内单倍型的平均遗传距离均无显著差异。再加上每个个体中异质mt-cox1序列的比例很低，这表明饥饿对有丝分裂型遗传多样性和线粒体功能的影响有限。然而，饥饿可以显著增加含有特定突变的单倍型的序列数量，对于线粒体异质性水平高于正常喂养组雌性的雄性，饥饿可以进一步增加它们的线粒体异质性。
In animals, starvation can increase the level of reactive oxygen species (ROS) in some tissues. Mitochondrial DNA (mtDNA) is more vulnerable to being attacked by ROS due to the lack of histone protection, leading to oxidative damage. However, whether starvation is associated with the genetic diversity of mtDNA remains unclear. Here, by using adult individuals of Drosophila melanogaster under three different feeding treatments (starvation, with the provision of only water, and normal feeding), based on the high-throughput sequencing results of the PCR amplicons of the partial sequences of the mitochondrial gene cytochrome c oxidase subunit I (mt-cox1), no significant difference in the mean number of mitochondrial haplotypes and the mean genetic distance of haplotypes within individuals were identified between the three treatment groups. Coupled with the low proportion of heterogeneous mt-cox1 sequences within each individual, it suggested that starvation had a limited impact on mitotype genetic diversity and mitochondrial function. Nevertheless, starvation could significantly increase the sequence number of haplotypes containing specific mutations, and for males with higher levels of mitochondrial heteroplasmy than females in the normal feeding group, starvation could further increase their mitochondrial heteroplasmy.
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影响因子：
9.3
作者：
Harvald, Eva Bang;Sprenger, Richard R.;Frgeman, Nils J.
通讯作者：
Frgeman, Nils J.
DOI：
10.1073/pnas.95.5.2372
发表时间：
1998-03-03
影响因子：
11.1
作者：
Kann, LM;Rosenblum, EB;Rand, DM
通讯作者：
Rand, DM
影响因子：
1.3
作者：
Bowser, Matthew Lewis;Brassfield, Rebekah;Stone, Joel
通讯作者：
Stone, Joel
影响因子：
2.4
作者：
Boyle, Elizabeth E.;Etter, Ron J.
通讯作者：
Etter, Ron J.
影响因子：
2.7
作者：
Elbrecht, Vasco;Vamos, Ecaterina Edith;Leese, Florian
通讯作者：
Leese, Florian