Drosophila microbiota modulates host metabolic gene expression via IMD/NF-κB signaling.
Drosophila microbiota modulates host metabolic gene expression via IMD/NF-κB signaling.
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DOI：
10.1371/journal.pone.0094729
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Leulier F
中科院分区：
文献类型：
--
作者：
Erkosar B;Defaye A;Bozonnet N;Puthier D;Royet J;Leulier F
大多数后生动物与它们的肠道微生物群进行互惠互动。尽管最近取得了进展，微生物群对宿主生理产生有益影响的分子机制在很大程度上仍然没有得到表征。在这里，我们使用与由一组定义的细菌菌株组成的标准化微生物群相关的纯果蝇成年人来研究微生物群关联对其宿主转录组的影响。我们的研究结果表明，果蝇微生物群对中肠转录组有显着的影响，并促进参与宿主消化功能和初级代谢的基因的表达。我们将IMD/Relish信号通路确定为这种微生物群介导的转录反应的中央调节器，并且我们揭示了中肠对其有益微生物群和细菌病原体的反应之间的显着转录权衡。综上所述，我们的研究结果表明，微生物群协会加强宿主营养和宿主代谢状态，两个关键的生理参数影响主机健身。我们的工作为后续的机制研究铺平了道路，以揭示这些微生物群依赖的转录特征如何转化为宿主的生理益处。
Most metazoans engage in mutualistic interactions with their intestinal microbiota. Despite recent progress the molecular mechanisms through which microbiota exerts its beneficial influences on host physiology are still largely uncharacterized. Here we use axenic Drosophila melanogaster adults associated with a standardized microbiota composed of a defined set of commensal bacterial strains to study the impact of microbiota association on its host transcriptome. Our results demonstrate that Drosophila microbiota has a marked impact on the midgut transcriptome and promotes the expression of genes involved in host digestive functions and primary metabolism. We identify the IMD/Relish signaling pathway as a central regulator of this microbiota-mediated transcriptional response and we reveal a marked transcriptional trade-off between the midgut response to its beneficial microbiota and to bacterial pathogens. Taken together our results indicate that microbiota association potentiates host nutrition and host metabolic state, two key physiological parameters influencing host fitness. Our work paves the way to subsequent mechanistic studies to reveal how these microbiota-dependent transcriptional signatures translate into host physiological benefits.
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影响因子：
3.7
作者：
Ridley EV;Wong AC;Westmiller S;Douglas AE
通讯作者：
Douglas AE
DOI：
10.1073/pnas.1009906107
发表时间：
2010-11-16
影响因子：
11.1
作者：
Sharon, Gil;Segal, Daniel;Rosenberg, Eugene
通讯作者：
Rosenberg, Eugene
影响因子：
2.7
作者：
Okumura, T;Matsumoto, A;Murakami, R
通讯作者：
Murakami, R
影响因子：
--
作者：
Li L;Edgar BA;Grewal SS
通讯作者：
Grewal SS
影响因子：
8.8
作者：
Buchon, Nicolas;Osman, Dani;Lemaitre, Bruno
通讯作者：
Lemaitre, Bruno