BnaMPK3 Is a Key Regulator of Defense Responses to the Devastating Plant Pathogen Sclerotinia sclerotiorum in Oilseed Rape
BnaMPK3 Is a Key Regulator of Defense Responses to the Devastating Plant Pathogen Sclerotinia sclerotiorum in Oilseed Rape
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BnaMPK3 是油菜中毁灭性植物病原菌核盘菌防御反应的关键调节因子
DOI：
10.3389/fpls.2019.00091
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发表时间：
2019-02
影响因子：
5.6
通讯作者：
Tan Xiao-Li
中科院分区：
文献类型：
--
作者：
Wang Zheng;Bao Ling-Li;Zhao Feng-Yun;Tang Min-Qiang;Chen Ting;Li Yaoming;Wang Bing-Xu;Fu Benzhong;Fang Hedi;Li Guan-Ying;Cao Jun;Ding Li-Na;Zhu Ke-Ming;Liu Sheng-Yi;Tan Xiao-Li
油菜菌核病是一种传统上难以防治的病害，给油菜生产造成了巨大的经济损失。分子育种是控制烟草赤星病的重要策略之一，而筛选与烟草赤星病防御反应相关的重要基因是分子育种的关键。在这里，我们报告说，一个B。油菜丝裂原活化蛋白激酶基因BnaMPK 3在油菜抗硫杆菌病中起重要作用。油菜菌核病。BnaMPK 3在茎、花和叶中高度表达，其产物定位于细胞核。此外，BnaMPK 3对S.茉莉酸（JA）或乙烯生物合成前体（ET）处理对油菜菌核病菌的生长有抑制作用，而水杨酸（SA）或脱落酸处理对油菜菌核病菌的生长无抑制作用。此外，在B. napus和Nicotiana benthamiana对S. RNAi转基因植物的抗性降低，而RNAi转基因植物的抗性降低。继S.当与野生型植物中的那些相比时，在BnaMPK 3-OE植物中与ET、JA和SA信号传导相关的防御反应增强，但在BnaMPK 3-RNAi植物中减弱;相比之下，H2 O2积累和细胞死亡的水平表现出相反的模式。候选基因关联分析表明，BnaMPK 3基因编码的BnaA 06 g18440 D位点是引起抗赤霉病变异的原因之一。天然B中的核盘菌。油菜种群这些结果表明BnaMPK 3是S.对油菜及相关经济作物的抗病性改良具有指导意义。
The disease caused by Sclerotinia sclerotiorum has traditionally been difficult to control, resulting in tremendous economic losses in oilseed rape (Brassica napus). Identification of important genes in the defense responses is critical for molecular breeding, an important strategy for controlling the disease. Here, we report that a B. napus mitogen-activated protein kinase gene, BnaMPK3, plays an important role in the defense against S. sclerotiorum in oilseed rape. BnaMPK3 is highly expressed in the stems, flowers and leaves, and its product is localized in the nucleus. Furthermore, BnaMPK3 is highly responsive to infection by S. sclerotiorum and treatment with jasmonic acid (JA) or the biosynthesis precursor of ethylene (ET), but not to treatment with salicylic acid (SA) or abscisic acid. Moreover, overexpression (OE) of BnaMPK3 in B. napus and Nicotiana benthamiana results in significantly enhanced resistance to S. sclerotiorum, whereas resistance is diminished in RNAi transgenic plants. After S. sclerotiorum infection, defense responses associated with ET, JA, and SA signaling are intensified in the BnaMPK3-OE plants but weakened in the BnaMPK3-RNAi plants when compared to those in the wild type plants; by contrast the level of both H2O2 accumulation and cell death exhibits a reverse pattern. The candidate gene association analyses show that the BnaMPK3-encoding BnaA06g18440D locus is a cause of variation in the resistance to S. sclerotiorum in natural B. napus population. These results suggest that BnaMPK3 is a key regulator of multiple defense responses to S. sclerotiorum, which may guide the resistance improvement of oilseed rape and related economic crops.
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影响因子：
5.6
作者：
M. Torruella;M. Flawiá;C. Eisenschlos;L. M. Vedia;C. Rubinstein;H. Torres
通讯作者：
M. Torruella;M. Flawiá;C. Eisenschlos;L. M. Vedia;C. Rubinstein;H. Torres
油菜核盘菌部分抗性相关位点的遗传分析（Brassica napus L.）
DOI：
10.1007/s00122-002-1171-2
发表时间：
2003-02
期刊：
Theor. Appl. GenetSCI源刊物,
影响因子：
--
作者：
赵建伟
通讯作者：
赵建伟
DOI：
10.1007/s11032-013-9954-5
发表时间：
2014
期刊：
Molecular breeding : new strategies in plant improvement
影响因子：
--
作者：
Wells R;Trick M;Soumpourou E;Clissold L;Morgan C;Werner P;Gibbard C;Clarke M;Jennaway R;Bancroft I
通讯作者：
Bancroft I
Plant-mPLoc：增强植物蛋白亚细胞定位预测能力的自上而下策略
影响因子：
3.7
作者：
Chou KC;Shen HB
通讯作者：
Shen HB
影响因子：
3.5
作者：
Guo, Xiaomei;Stotz, Henrik U.
通讯作者：
Stotz, Henrik U.