Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?
Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?
复制标题
DOI：
10.3390/ijerph14091006
复制
发表时间：
2017-09-02
影响因子：
--
通讯作者：
Rasgon JL
中科院分区：
文献类型：
--
作者：
Macias VM;Ohm JR;Rasgon JL
蚊媒病原体给人类健康带来巨大负担。现有的工具包不足以支持目前为实现消除和根除疾病目标而开展的病媒控制工作。遗传方法可能为蚊子控制增加一套重要工具的观点并不新鲜，但最近CRISPR/Cas9系统在位点特异性基因编辑方面的改进增强了我们使用反向遗传学研究蚊子生物学和生产基于遗传学的工具的能力。Cas9介导的基因编辑是基因驱动策略的有效且适应性强的平台，其在将所需的抑制和病原体阻断基因型渗入野生蚊子种群中方面具有优于侵入性释放策略的优势;直到最近，有效的基因驱动在很大程度上还遥不可及。许多考虑因素将为有效使用新的遗传工具，包括基因驱动提供信息。在这里，我们回顾了蚊子生物学遗传进步的漫长历史，并讨论了有效的位点特异性基因编辑对载体生物学的影响，以及由此产生的部署新的遗传工具以减少蚊媒疾病的潜力。
Mosquito-borne pathogens place an enormous burden on human health. The existing toolkit is insufficient to support ongoing vector-control efforts towards meeting disease elimination and eradication goals. The perspective that genetic approaches can potentially add a significant set of tools toward mosquito control is not new, but the recent improvements in site-specific gene editing with CRISPR/Cas9 systems have enhanced our ability to both study mosquito biology using reverse genetics and produce genetics-based tools. Cas9-mediated gene-editing is an efficient and adaptable platform for gene drive strategies, which have advantages over innundative release strategies for introgressing desirable suppression and pathogen-blocking genotypes into wild mosquito populations; until recently, an effective gene drive has been largely out of reach. Many considerations will inform the effective use of new genetic tools, including gene drives. Here we review the lengthy history of genetic advances in mosquito biology and discuss both the impact of efficient site-specific gene editing on vector biology and the resulting potential to deploy new genetic tools for the abatement of mosquito-borne disease.
登录
查看更多内容
影响因子：
4.6
作者：
Awata H;Watanabe T;Hamanaka Y;Mito T;Noji S;Mizunami M
通讯作者：
Mizunami M
影响因子：
3.8
作者：
Beaty, Barry J.;Prager, Denis J.;Kafatos, Fotis C.
通讯作者：
Kafatos, Fotis C.
影响因子：
3.3
作者：
Arensburger, P;Kim, YJ;Atkinson, PW
通讯作者：
Atkinson, PW
影响因子：
3
作者：
Ageep, Tellal B.;Damiens, David;El Sayed, Badria B.
通讯作者：
El Sayed, Badria B.
影响因子：
9.2
作者：
Akbari, Omar S.;Matzen, Kelly D.;Hay, Bruce A.
通讯作者：
Hay, Bruce A.