Lowered sensitivity of bitter taste receptors to β-glucosides in bamboo lemurs: an instance of parallel and adaptive functional decline in TAS2R16?
Lowered sensitivity of bitter taste receptors to β-glucosides in bamboo lemurs: an instance of parallel and adaptive functional decline in TAS2R16?
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DOI：
10.1098/rspb.2021.0346
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发表时间：
2021-04-14
期刊：
影响因子：
--
通讯作者：
Imai H
中科院分区：
文献类型：
--
作者：
Itoigawa A;Fierro F;Chaney ME;Lauterbur ME;Hayakawa T;Tosi AJ;Niv MY;Imai H
苦味有助于检测潜在的有害物质，并通过脊椎动物舌头和口腔上表达的苦味受体（TAS 2 R）感知。在灵长类动物中，TAS 2 R16特异性识别β-葡萄糖苷，这在生氰植物使用氰化物作为摄食威慑物中很重要。在本研究中，我们进行了基于细胞的功能测定，以研究TAS 2 R16对β-葡萄糖苷的敏感性在三种竹狐猴（Prolemur simus，Hapalemur aureus和H. griseus），主要食用高氰化物的竹子。来自竹狐猴的TAS 2 R16受体对β-葡萄糖苷（包括生氰葡萄糖苷）的敏感性低于密切相关的环尾狐猴（Lemur catta）。竹狐猴最后共同祖先（LCA）和Hapalemur LCA的TAS 2 R16的祖先重建显示，环尾狐猴和竹狐猴对β-葡萄糖苷的敏感性介于两者之间。突变分析表明，P. simus和H. griseus有单独的物种特异性取代，导致敏感性降低。这些结果表明，在细胞水平上对β-葡萄糖苷的低敏感性-一个潜在的适应性特征，以生氰竹为食-独立进化后，Prolemur-Hapalemur分裂在每个物种。
Bitter taste facilitates the detection of potentially harmful substances and is perceived via bitter taste receptors (TAS2Rs) expressed on the tongue and oral cavity in vertebrates. In primates, TAS2R16 specifically recognizes β-glucosides, which are important in cyanogenic plants' use of cyanide as a feeding deterrent. In this study, we performed cell-based functional assays for investigating the sensitivity of TAS2R16 to β-glucosides in three species of bamboo lemurs (Prolemur simus, Hapalemur aureus and H. griseus), which primarily consume high-cyanide bamboo. TAS2R16 receptors from bamboo lemurs had lower sensitivity to β-glucosides, including cyanogenic glucosides, than that of the closely related ring-tailed lemur (Lemur catta). Ancestral reconstructions of TAS2R16 for the bamboo-lemur last common ancestor (LCA) and that of the Hapalemur LCA showed an intermediate sensitivity to β-glucosides between that of the ring-tailed lemurs and bamboo lemurs. Mutagenetic analyses revealed that P. simus and H. griseus had separate species-specific substitutions that led to reduced sensitivity. These results indicate that low sensitivity to β-glucosides at the cellular level—a potentially adaptive trait for feeding on cyanogenic bamboo—evolved independently after the Prolemur–Hapalemur split in each species.
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影响因子：
14.9
作者：
Kooistra AJ;Mordalski S;Pándy-Szekeres G;Esguerra M;Mamyrbekov A;Munk C;Keserű GM;Gloriam DE
通讯作者：
Gloriam DE
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
30.8
作者：
Bufe, B;Hofmann, T;Meyerhof, W
通讯作者：
Meyerhof, W
影响因子：
9.2
作者：
Eronen, Jussi T.;Zohdy, Sarah;Jernvall, Jukka
通讯作者：
Jernvall, Jukka
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM