Mirrored pyramidal wells for simultaneous multiple vantage point microscopy.
Mirrored pyramidal wells for simultaneous multiple vantage point microscopy.
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DOI：
10.1111/j.1365-2818.2008.02110.x
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发表时间：
2008-10
影响因子：
2
通讯作者：
Wikswo JP
中科院分区：
文献类型：
--
作者：
Seale KT;Reiserer RS;Markov DA;Ges IA;Wright C;Janetopoulos C;Wikswo JP
We report a novel method for obtaining simultaneous images from multiple vantage points of a microscopic specimen using size-matched microscopic mirrors created from anisotropically etched silicon. The resulting pyramidal wells enable bright-field and fluorescent side-view images, and when combined with z-sectioning, provide additional information for 3D reconstructions of the specimen. We have demonstrated the 3D localization and tracking over time of the centrosome of a live Dictyostelium discoideum. The simultaneous acquisition of images from multiple perspectives also provides a five-fold increase in the theoretical collection efficiency of emitted photons, a property which may be useful for low-light imaging modalities such as bioluminescence, or low abundance surface-marker labelling.
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