A ROP GTPase-dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots.
A ROP GTPase-dependent auxin signaling pathway regulates the subcellular distribution of PIN2 in Arabidopsis roots.
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DOI：
10.1016/j.cub.2012.05.019
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发表时间：
2012-07-24
期刊：
影响因子：
9.2
通讯作者：
Yang, Zhenbiao
中科院分区：
文献类型：
--
作者：
Lin, Deshu;Nagawa, Shingo;Chen, Jisheng;Cao, Lingyan;Chen, Xu;Xu, Tongda;Li, Hongjiang;Dhonukshe, Pankaj;Yamamuro, Chizuko;Friml, Jiri;Scheres, Ben;Fu, Ying;Yang, Zhenbiao
PIN蛋白介导的生长素极性运输对植物的发育、格局形成和形态建成至关重要。生长素通过抑制PIN内吞作用参与生长素极性运输的调节，但生长素如何调节这一过程尚不清楚。我们的遗传筛选确定了拟南芥SPIKE 1（SPK 1）基因，其功能丧失突变增加侧根密度和延迟向重力反应，如pin 2敲除突变。SPK 1属于Rho鸟嘌呤核苷酸交换因子（RhoGEFs）的保守的DHR 2-DOCK家族。spk 1突变诱导的PIN 2内化不受生长素抑制，ROP 6 GT3或其效应子RIC 1的功能丧失突变也是如此。此外，SPK 1是生长素诱导ROP 6活化所必需的。我们的研究结果建立了一个Rho GTP酶为基础的生长素信号通路，通过抑制其在拟南芥根中的内化来维持PIN 2极性分布到质膜。我们的研究结果提供了新的见解信号传导机制，基础上的长距离生长素运输所需的PIN的动态贩运的监管和连接生长素信号PIN介导的图案形成和形态发生。
PIN protein-mediated auxin polar transport is critically important for development, pattern formation, and morphogenesis in plants. Auxin has been implicated in the regulation of polar auxin transport by inhibiting PIN endocytosis, but how auxin regulates this process is poorly understood. Our genetic screen identified the Arabidopsis SPIKE1 (SPK1) gene whose loss-of-function mutations increased lateral root density and retarded gravitropic responses, as do pin2 knockout mutations. SPK1 belongs to the conserved DHR2-DOCK family of Rho guanine nucleotide exchange factors (RhoGEFs). The spk1 mutations induced PIN2 internalization that was not suppressed by auxin, as did the loss-of-function mutations for ROP6 GTPase or its effector RIC1. Furthermore, SPK1 was required for auxin induction of ROP6 activation. Our results have established a Rho GTPase-based auxin signaling pathway that maintains PIN2 polar distribution to the plasma membrane via inhibition of its internalization in Arabidopsis roots. Our findings provide new insights into signaling mechanisms that underlie the regulation of the dynamic trafficking of PINs required for long-distance auxin transport and that link auxin signaling to PIN-mediated pattern formation and morphogenesis.
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影响因子：
9.8
作者：
Nagawa S;Xu T;Lin D;Dhonukshe P;Zhang X;Friml J;Scheres B;Fu Y;Yang Z
通讯作者：
Yang Z
影响因子：
64.5
作者：
Geldner, N;Anders, N;Jürgens, G
通讯作者：
Jürgens, G
影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
DOI：
10.1016/j.cub.2009.08.052
发表时间：
2009-11-17
期刊：
Current biology : CB
影响因子：
--
作者：
Fu Y;Xu T;Zhu L;Wen M;Yang Z
通讯作者：
Yang Z
影响因子：
9.8
作者：
Hazak O;Bloch D;Poraty L;Sternberg H;Zhang J;Friml J;Yalovsky S
通讯作者：
Yalovsky S