Melatonin Regulates Root Meristem by Repressing Auxin Synthesis and Polar Auxin Transport in Arabidopsis.
Melatonin Regulates Root Meristem by Repressing Auxin Synthesis and Polar Auxin Transport in Arabidopsis.
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DOI：
10.3389/fpls.2016.01882
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发表时间：
2016
影响因子：
5.6
通讯作者：
He C
中科院分区：
文献类型：
--
作者：
Wang Q;An B;Wei Y;Reiter RJ;Shi H;Luo H;He C
褪黑素（N-乙酰基-5-甲氧基色胺）在调节生物和非生物胁迫耐受性、生物节律、植物生长和发育方面发挥着重要作用。褪黑激素和生长素共享相同的生物合成底物（色氨酸），在植物发育中也具有类似的作用。然而，褪黑素在调节植物根系生长中的具体功能以及褪黑素与生长素之间的关系及其潜在机制仍不清楚。在这项研究中，我们发现高浓度的褪黑激素通过减少根分生组织的大小而显着抑制拟南芥根的生长。进一步的研究表明，褪黑素对拟南芥中的生长素生物合成、PINFORMED (PIN) 蛋白的表达以及生长素反应产生负调控。此外，三重突变体pin1pin3pin7的根生长比野生型对褪黑激素处理的耐受性更强，这表明PIN1/3/7在褪黑激素介导的根生长中发挥着重要作用。褪黑激素和 5-三碘苯甲酸 (TIBA) 的联合处理并没有增强褪黑激素介导的根分生组织大小的减少，表明极性生长素转运 (PAT) 可能是褪黑激素处理调节根分生组织大小所必需的。总而言之，这项研究表明褪黑素至少部分通过生长素合成和极性生长素运输来调节拟南芥根部生长。
Melatonin (N-acetyl-5-methoxytryptamine) plays important roles in regulating both biotic and abiotic stress tolerance, biological rhythms, plant growth and development. Sharing the same substrate (tryptophan) for the biosynthesis, melatonin and auxin also have similar effects in plant development. However, the specific function of melatonin in modulating plant root growth and the relationship between melatonin and auxin as well as underlying mechanisms are still unclear. In this study, we found high concentration of melatonin remarkably inhibited root growth in Arabidopsis by reducing root meristem size. Further studies showed that melatonin negatively regulated auxin biosynthesis, the expression of PINFORMED (PIN) proteins as well as auxin response in Arabidopsis. Moreover, the root growth of the triple mutant pin1pin3pin7 was more tolerant than that of wild-type in response to melatonin treatment, suggesting the essential role of PIN1/3/7 in melatonin-mediated root growth. Combination treatment of melatonin and 5-Triiodobenzoic acid (TIBA) did not enhance melatonin-mediated reduction of root meristem size, indicating that polar auxin transport (PAT) may be necessary for the regulation of root meristem size by melatonin treatment. Taken together, this study indicates that melatonin regulates root growth in Arabidopsis, through auxin synthesis and polar auxin transport, at least partially.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
5.6
作者：
Carrillo-Vico A;Lardone PJ;Alvarez-Sánchez N;Rodríguez-Rodríguez A;Guerrero JM
通讯作者：
Guerrero JM
影响因子：
10.3
作者：
DUBBELS, R;REITER, RJ;SCHLOOT, W
通讯作者：
SCHLOOT, W
影响因子：
10.3
作者：
Kang, Kiyoon;Lee, Kyungjin;Back, Kyoungwhan
通讯作者：
Back, Kyoungwhan
影响因子：
10.5
作者：
Cheng, Youfa;Dai, Xinhua;Zhao, Yunde
通讯作者：
Zhao, Yunde