Effects of Chronic High-Frequency rTMS Protocol on Respiratory Neuroplasticity Following C2 Spinal Cord Hemisection in Rats.
Effects of Chronic High-Frequency rTMS Protocol on Respiratory Neuroplasticity Following C2 Spinal Cord Hemisection in Rats.
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DOI：
10.3390/biology11030473
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发表时间：
2022-03-19
期刊：
影响因子：
4.2
通讯作者：
Vinit S
中科院分区：
文献类型：
--
作者：
Michel-Flutot P;Jesus I;Vanhee V;Bourcier CH;Emam L;Ouguerroudj A;Lee KZ;Zholudeva LV;Lane MA;Mansart A;Bonay M;Vinit S
高位脊髓损伤(SCI)会导致永久性的横隔膜瘫痪，并在颈髓损伤后引发有害的创伤后炎症过程。我们使用了一种非侵入性的治疗工具(重复经颅磁刺激(RTMS))，以利用备用下行呼吸道的可塑性，减少炎症过程。简而言之，本研究的结果表明，慢性高频rTMS可以改善呼吸功能障碍，并通过减少脊髓损伤后脊髓内有害的创伤后炎症过程而诱导神经元可塑性。因此，这种治疗工具可以被采用和/或与其他治疗干预措施相结合，以进一步提高有益的结果。高位脊髓损伤(SCI)会导致永久性的横隔膜瘫痪。寻找治疗方法来诱导功能性运动恢复是必不可少的。重复经颅磁刺激(RTMS)是一种有前途的非侵入性治疗工具，可以利用备用下行呼吸道的可塑性。在这里，我们测试了慢性高频(10赫兹)rTMS在C2半切大鼠皮层上方施加7天、1个月或2个月时的效果。应用rTMS 1个月后，完整的横隔肌肌电(EMG)活性和兴奋性(横隔膜运动诱发电位)均增加。有趣的是，尽管rTMS治疗对呼吸暂停期间受损的横隔膜活动没有真正的功能影响，但2个月的rTMS治疗加强了现有的交叉膈神经通路，使受损的横隔膜在呼吸挑战(即窒息)时增加其活动。这一效应可能是由于腹外侧束旁核呼吸下行纤维的增强(GAP-43阳性纤维的增加)，维持在C1-C3脊髓的炎症减轻(CD68和IBA1标记减少)，以及慢性rTMS治疗后膈运动神经元细胞内可塑性过程的加速。这些结果表明，慢性高频rTMS可以改善呼吸功能障碍，并通过减少脊髓损伤后颈髓有害的创伤后炎症过程而诱导神经元可塑性。因此，这种治疗工具可以被采用和/或与其他治疗干预措施相结合，以进一步提高有益的结果。
High spinal cord injuries (SCIs) are known to lead to permanent diaphragmatic paralysis, and to induce deleterious post-traumatic inflammatory processes following cervical spinal cord injury. We used a noninvasive therapeutic tool (repetitive transcranial magnetic stimulation (rTMS)), to harness plasticity in spared descending respiratory circuit and reduce the inflammatory processes. Briefly, the results obtained in this present study suggest that chronic high-frequency rTMS can ameliorate respiratory dysfunction and elicit neuronal plasticity with a reduction in deleterious post-traumatic inflammatory processes in the cervical spinal cord post-SCI. Thus, this therapeutic tool could be adopted and/or combined with other therapeutic interventions in order to further enhance beneficial outcomes. High spinal cord injuries (SCIs) lead to permanent diaphragmatic paralysis. The search for therapeutics to induce functional motor recovery is essential. One promising noninvasive therapeutic tool that could harness plasticity in a spared descending respiratory circuit is repetitive transcranial magnetic stimulation (rTMS). Here, we tested the effect of chronic high-frequency (10 Hz) rTMS above the cortical areas in C2 hemisected rats when applied for 7 days, 1 month, or 2 months. An increase in intact hemidiaphragm electromyogram (EMG) activity and excitability (diaphragm motor evoked potentials) was observed after 1 month of rTMS application. Interestingly, despite no real functional effects of rTMS treatment on the injured hemidiaphragm activity during eupnea, 2 months of rTMS treatment strengthened the existing crossed phrenic pathways, allowing the injured hemidiaphragm to increase its activity during the respiratory challenge (i.e., asphyxia). This effect could be explained by a strengthening of respiratory descending fibers in the ventrolateral funiculi (an increase in GAP-43 positive fibers), sustained by a reduction in inflammation in the C1–C3 spinal cord (reduction in CD68 and Iba1 labeling), and acceleration of intracellular plasticity processes in phrenic motoneurons after chronic rTMS treatment. These results suggest that chronic high-frequency rTMS can ameliorate respiratory dysfunction and elicit neuronal plasticity with a reduction in deleterious post-traumatic inflammatory processes in the cervical spinal cord post-SCI. Thus, this therapeutic tool could be adopted and/or combined with other therapeutic interventions in order to further enhance beneficial outcomes.
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影响因子：
5.3
作者：
Doperalski, N. J.;Fuller, D. D.
通讯作者：
Fuller, D. D.
脊髓损伤后经脊髓磁刺激的再生效应： 脊髓损伤后的再生效应：效应的机制和途径。
DOI：
10.1007/s13311-020-00915-5
发表时间：
2020-10
期刊：
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics
影响因子：
--
作者：
Chalfouh C;Guillou C;Hardouin J;Delarue Q;Li X;Duclos C;Schapman D;Marie JP;Cosette P;Guérout N
通讯作者：
Guérout N
影响因子：
5.3
作者：
Gersner, Roman;Kravetz, Elena;Zangen, Abraham
通讯作者：
Zangen, Abraham
影响因子：
3.5
作者：
Cullen CL;Young KM
通讯作者：
Young KM
影响因子：
1.7
作者：
Kozel, F. Andrew
通讯作者：
Kozel, F. Andrew