Axonal guidance in the chicken retina.
Axonal guidance in the chicken retina.
复制标题
鸡视网膜中的轴突引导。
作者：
H. Stier;Burkhard Schlosshauer
During retina development, ganglion cells extend their axons exclusively into the innermost tissue layer, but not into outer retina layers. In order to elucidate guiding mechanisms for axons, tissue strips of embryonic chicken retinae were explanted onto retinal cryosections (cryoculture). Ganglion cell axons originating from the explant grew preferentially on the innermost retina layer of cryosections, whereas outer tissue layers were avoided, very much as in vivo. Stereotropism, interaction with laminin of the basal lamina and axonal fasciculation did not significantly affect oriented axonal outgrowth, so that stereotropism as a guidance mechanism could be excluded. Ganglion cell axons were not directed by physical barriers, e.g. microstructured silicon oxide chips. Similarly, UV induced protein inactivation revealed that laminin present in the inner retina did not provide a guidance cue. Even in the absence of ganglion cell axons in retinal cryosections due to prior optic nerve transection in ovo, the growth preference for the innermost retina layer was maintained in cryocultures. However, oriented elongation of axons along the innermost retina layer was lost when radial glial endfeet were selectively eliminated in retinal cryosections. In addition, glial endfeet provided an excellent growth substratum when pure preparations of endfeet were employed in explant cultures. The preference for glial endfeet positioned at the inner retina surface was accompanied by the avoidance of outer retina layers, most likely because of inhibitory components in this region. This assumption is corroborated by the finding that addition of exogenous growth-promoting laminin to cryosections did not abolish the inhibition. Laminin on glass surfaces provided an excellent substratum. Axonal outgrowth was also seriously hampered on specifically purified cells of the outer retina. Most notable, however, in cryocultures aberrant innervation of outer retina layers could be induced by prior heat or protease treatment of cryosections, which pointed to proteins as potential inhibitory components. In summary the data substantiate the hypothesis that within the retina, ganglion cell axons are guided by a dual mechanism based on a permissive and an inhibitory zone. Radial glia is likely to be instructive in this process.
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通过神经上皮末足上预先形成的粘附通路对体内视轴突进行引导。
影响因子：
2.7
作者：
Silver,J;Rutishauser,U
通讯作者：
Rutishauser,U
靶源性星形胶质细胞以区域特异性方式调节轴突生长。
影响因子：
2.7
作者：
Qian,JA;Bull,MS;Levitt,P
通讯作者：
Levitt,P
形态调节分子对细胞粘附的介导和抑制。
DOI：
10.1101/sqb.1992.057.01.036
发表时间：
1992
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Edelman,GM
通讯作者：
Edelman,GM
DOI：
10.1002/neu.480230311
发表时间：
1992-04-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
SNOW, DM;LETOURNEAU, PC
通讯作者：
LETOURNEAU, PC
硫酸软骨素蛋白多糖可能影响视网膜神经节细胞生长的方向。
DOI：
10.1242/dev.113.4.1473
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Snow,DM;Watanabe,M;Letourneau,PC;Silver,J
通讯作者：
Silver,J