Identification of a microtubule-associated motor protein essential for dendritic differentiation.
Identification of a microtubule-associated motor protein essential for dendritic differentiation.
复制标题
鉴定微管相关的运动蛋白对于树突分化必不可少。
DOI：
10.1083/jcb.138.4.833
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发表时间：
1997-08-25
影响因子：
7.8
通讯作者：
Baas, PW
中科院分区：
文献类型：
--
作者：
Sharp, DJ;Yu, WQ;Ferhat, L;Kuriyama, R;Rueger, DC;Baas, PW
树枝状微管阵列的典型特征是其极性取向的不均匀图案。在树突的发育过程中，首先出现一组正端-远端微管，然后这些微管被一组相反取向的微管连接起来。我们实验室的研究表明，在发育的关键阶段，后一种微管通过它们从神经元细胞体的特定运输而嵌入到微管阵列中(Sharp，D.J.，W.Yu，和P.W.Baas)。1995年。J.细胞生物学。130：93-104)。此外，我们还证实了被称为CHO1/MKLP1的有丝分裂运动蛋白具有以这种方式运输微管的适当性质(Sharp，D.J.，R.Kuriyama和P.W.Baas)。1996年。J.神经学16：4370-4375)。在本研究中，我们试图确定CHO1/MKLP1是否继续在终末有丝分裂后神经元中表达，以及它是否是建立树突状微管阵列所必需的。原位杂交分析表明，CHO1/MKLP1在有丝分裂后培养的大鼠交感神经元和海马神经元中均有表达。免疫荧光分析表明，马达没有轴突，但富含发育中的树突，表现为与微管阵列相关的离散斑块。用CHO1/MKLP1的反义寡核苷酸处理神经元，可能是通过抑制其非均匀微管极性模式的建立来抑制树突状分化。我们的结论是，CHO1/MKLP1将微管从细胞体运输到发育中的树突，其负端领先，从而建立了树突不均匀的微管极性模式。
The quintessential feature of the dendritic microtubule array is its nonuniform pattern of polarity orientation. During the development of the dendrite, a population of plus end–distal microtubules first appears, and these microtubules are subsequently joined by a population of oppositely oriented microtubules. Studies from our laboratory indicate that the latter microtubules are intercalated within the microtubule array by their specific transport from the cell body of the neuron during a critical stage in development (Sharp, D.J., W. Yu, and P.W. Baas. 1995. J. Cell Biol. 130:93– 104). In addition, we have established that the mitotic motor protein termed CHO1/MKLP1 has the appropriate properties to transport microtubules in this manner (Sharp, D.J., R. Kuriyama, and P.W. Baas. 1996. J. Neurosci. 16:4370–4375). In the present study we have sought to determine whether CHO1/MKLP1 continues to be expressed in terminally postmitotic neurons and whether it is required for the establishment of the dendritic microtubule array. In situ hybridization analyses reveal that CHO1/MKLP1 is expressed in postmitotic cultured rat sympathetic and hippocampal neurons. Immunofluorescence analyses indicate that the motor is absent from axons but is enriched in developing dendrites, where it appears as discrete patches associated with the microtubule array. Treatment of the neurons with antisense oligonucleotides to CHO1/MKLP1 suppresses dendritic differentiation, presumably by inhibiting the establishment of their nonuniform microtubule polarity pattern. We conclude that CHO1/MKLP1 transports microtubules from the cell body into the developing dendrite with their minus ends leading, thereby establishing the nonuniform microtubule polarity pattern of the dendrite.
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DOI：
10.1083/jcb.130.1.93
发表时间：
1995-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sharp DJ;Yu W;Baas PW
通讯作者：
Baas PW
影响因子：
64.8
作者：
NISLOW, C;LOMBILLO, VA;MCINTOSH, JR
通讯作者：
MCINTOSH, JR
影响因子：
64.8
作者：
Heald, R;Tournebize, R;Karsenti, E
通讯作者：
Karsenti, E
DOI：
10.1083/jcb.109.6.3085
发表时间：
1989-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Baas PW;Black MM;Banker GA
通讯作者：
Banker GA
影响因子：
64.8
作者：
BAMBURG, JR;BRAY, D;CHAPMAN, K
通讯作者：
CHAPMAN, K