Genome-Wide Transcriptional and Post-transcriptional Regulation of Innate Immune and Defense Responses of Bovine Mammary Gland to Staphylococcus aureus.
Genome-Wide Transcriptional and Post-transcriptional Regulation of Innate Immune and Defense Responses of Bovine Mammary Gland to Staphylococcus aureus.
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牛乳腺对金黄色葡萄球菌的先天免疫和防御反应的全基因组转录和转录后调节
DOI：
10.3389/fcimb.2016.00193
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发表时间：
2016
影响因子：
5.7
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Fang L;Hou Y;An J;Li B;Song M;Wang X;Sørensen P;Dong Y;Liu C;Wang Y;Zhu H;Zhang S;Yu Y
金黄色葡萄球菌(S.金黄色葡萄球菌）对哺乳动物和公众健康是个问题。了解寄主对S.金黄色葡萄球菌仍然难以捉摸。本研究以牛乳腺细胞为研究对象，在高、低浓度的沙门氏菌乳房内感染（IMI）后24 h，检测了乳腺细胞中mRNA和miRNA的全基因组表达。金黄色。与阴性对照组相比，在高浓度（109 cfu/mL）的S-p57菌群中鉴定出194个高可信度的应答基因。金黄色葡萄球菌，其主要涉及与先天免疫系统有关的途径和生物学过程，例如细胞因子-细胞因子受体相互作用和炎症反应。相比之下，只有21个高置信度基因在低浓度（106 cfu/mL）的S.金黄色葡萄球菌，其轻微干扰细胞信号传导并引起相应的反应，如血管收缩，表明有限的干扰和免疫逃避。此外，在高浓度S.金黄色。网络分析表明，这两种miRNA可能通过抑制CXCL 14和KIT在宿主防御细菌感染中发挥重要作用。在感染S. coli 24 h后，牛乳腺上皮细胞中CXCL 14表达显著下调。金黄色葡萄球菌（108 cfu/mL）。与QTL数据库的整合分析进一步表明，CXCL 14、KIT和SLC 4A 11）作为牛乳腺炎的候选物。本研究首次系统地揭示了牛乳腺细胞对S.金黄色葡萄球菌呈剂量依赖性模式，并强调了miRNA-基因-途径相互作用网络中复杂应答机制。
Staphylococcus aureus (S. aureus) is problematic for lactating mammals and public health. Understanding of mechanisms by which the hosts respond to severe invasion of S. aureus remains elusive. In this study, the genome-wide expression of mRNAs and miRNAs in bovine mammary gland cells were interrogated at 24 h after intra-mammary infection (IMI) with high or low concentrations of S. aureus. Compared to the negative control quarters, 194 highly-confident responsive genes were identified in the quarters with high concentration (109 cfu/mL) of S. aureus, which were predominantly implicated in pathways and biological processes pertaining to innate immune system, such as cytokine-cytokine receptor interaction and inflammatory response. In contrast, only 21 highly-confident genes were significantly differentially expressed in face of low concentration (106 cfu/mL) of S. aureus, which slightly perturbed the cell signaling and invoked corresponding responses like vasoconstriction, indicating limited perturbations and immunological evading. Additionally, the significant up-regulations of bta-mir-223 and bta-mir-21-3p were observed in the quarters infected by high concentration of S. aureus. Network analysis suggested that the two miRNAs' pivotal roles in defending hosts against bacterial infection probably through inhibiting CXCL14 and KIT. The significant down-regulation of CXCL14 was also observed in bovine mammary epithelial cells at 24 h post-infection of S. aureus (108 cfu/mL) in vitro. Integrated analysis with QTL database further suggested 28 genes (e.g., CXCL14, KIT, and SLC4A11) as candidates of bovine mastitis. This study first systematically revealed transcriptional and post-transcriptional responses of bovine mammary gland cells to invading S. aureus in a dosage-dependent pattern, and highlighted a complicated responsive mechanism in a network of miRNA-gene-pathway interplay.
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影响因子：
3.1
作者：
Gombart AF
通讯作者：
Gombart AF
影响因子：
3.5
作者：
De Oliveira, AP;Watts, JL;Aerestrup, FM
通讯作者：
Aerestrup, FM
影响因子：
5.5
作者：
Gombart, AF;Krug, U;Koeffler, HP
通讯作者：
Koeffler, HP
影响因子：
4.4
作者：
Jin W;Ibeagha-Awemu EM;Liang G;Beaudoin F;Zhao X;Guan le L
通讯作者：
Guan le L
MicroRNA解剖的生物信息学工具。
影响因子：
14.9
作者：
Akhtar MM;Micolucci L;Islam MS;Olivieri F;Procopio AD
通讯作者：
Procopio AD