Rapid detection of CO in vitro and in vivo with a ratiometric probe showing near-infrared turn-on fluorescence, large Stokes shift, and high signal-to-noise ratio
Rapid detection of CO in vitro and in vivo with a ratiometric probe showing near-infrared turn-on fluorescence, large Stokes shift, and high signal-to-noise ratio
复制标题
使用比例探针快速检测体外和体内 CO，显示近红外开启荧光、大斯托克斯位移和高信噪比
DOI：
10.1016/j.snb.2019.127075
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发表时间：
2019-12
期刊：
影响因子：
--
通讯作者：
Feng Guoqiang
中科院分区：
文献类型：
--
作者：
Zhou Enbo;Gong Shengyi;Feng Guoqiang
研制了一种新的近红外(NIR)荧光探针，用于检测活体系统中的CO。该探针基于一种独特的氰基荧光团，并使用烯丙基醚部分作为反应位点。在溶液中的光学研究表明，该探针具有良好的水溶性，对CO具有良好的传感性能，包括快速响应、高选择性和高灵敏度、大的斯托克斯位移(123 Nm)、明显的比色变化和在715 nm处显著开启的高信噪比的近红外荧光变化。用该探针在715 nm和570 nm处对CO进行比率荧光检测也是适用的。传感机理被证明是一个通过Tsuji-Trost反应去除烯丙基来恢复荧光团的过程。此外，该探针用于活体细胞和动物体内一氧化碳的检测，具有良好的生物成像性能。此外，该探针表明，在氧化应激条件下，可以产生更多的CO。这些结果表明，该探针有望成为体内和体外检测CO的一种新工具。
A new near-infrared (NIR) fluorescent probe was developed for the detection of CO in living systems. This probe is based on a unique cyanine fluorophore and uses an allyl ether moiety as the reaction site. Optical studies in solution show that this probe has good water solubility and possesses desirable sensing properties for CO including rapid response, high selectivity and sensitivity, large Stokes shift (123 nm), distinct colorimetric changes, and significant turn-on NIR fluorescence changes at 715 nm with high signal-to-noise ratio. Ratiometric fluorescent detection of CO with this probe at 715 and 570 nm is also applicable. The sensing mechanism was proven to be a process of recovery of the fluorophore by removing the allyl group via Tsuji-Trost reaction. Furthermore, this probe was applied for detecting CO in living cells and animals with good bioimaging performance. Moreover, indicated by this probe, more CO could be produced under oxidative stress conditions. All the results indicate that this probe can be applied as a promising new tool for in vitro and in vivo detection of CO.
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