Spatially Resolving Size Effects on Diffusivity in Nanoporous Extracellular Matrix-like Materials with Fluorescence Correlation Spectroscopy Super-Resolution Optical Fluctuation Imaging.
Spatially Resolving Size Effects on Diffusivity in Nanoporous Extracellular Matrix-like Materials with Fluorescence Correlation Spectroscopy Super-Resolution Optical Fluctuation Imaging.
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DOI：
10.1021/acs.jpcb.3c00941
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发表时间：
2023-05-25
影响因子：
3.3
通讯作者：
Kisley, Lydia
中科院分区：
文献类型：
--
作者：
Chatterjee, Surajit;Kramer, Stephanie N.;Wellnitz, Benjamin;Kim, Albert;Kisley, Lydia
研究表明，多孔微环境中的纳米结构对分子的扩散动力学有很大的影响。然而，环境和分子之间的相互作用如何影响扩散动力学还没有得到深入的研究。在这里，我们展示了荧光相关光谱超分辨光学涨落成像(FcsSOFI)可以用来精确测量分子在不同基质中的扩散动力学，例如纳米图案表面和多孔琼脂糖水凝胶。我们的数据证明了fcsSOFI的稳健性，它不仅可以量化分子在非均质介质中的扩散速度，而且可以恢复分辨率在100 nm数量级的基质结构。对于不同大小的葡聚糖分子，我们发现扩散系数对分子流体动力学半径的变化很敏感。FcsSOFI图像进一步显示，较小的葡聚糖分子可以自由地通过水凝胶的小孔，并报告了平均扩散系数没有捕捉到的详细的孔结构和局部扩散不均匀。相反，较大的葡聚糖分子受到限制，无法在水凝胶中自由移动，只突出较大的孔结构。这些发现使fcsSOFI成为表征生物悬浮剂基质中不同大分子的空间和扩散信息的强大工具。
It is well documented that the nanoscale structures within porous microenvironments greatly impact the diffusion dynamics of molecules. However, how the interaction between the environment and molecules influences the diffusion dynamics has not been thoroughly explored. Here, we show that fluorescence correlation spectroscopy super-resolution optical fluctuation imaging (fcsSOFI) can be used to accurately measure the diffusion dynamics of molecules within varying matrices such as nano-patterned surfaces and porous agarose hydrogels. Our data demonstrate the robustness of fcsSOFI, where it is possible not only to quantify the diffusion speeds of molecules in heterogeneous media but also to recover the matrix structure with resolution on the order of 100 nm. Using dextran molecules of varying sizes, we show that the diffusion coefficient is sensitive to the change in the molecular hydrodynamic radius. fcsSOFI images further reveal that smaller dextran molecules can freely move through the small pores of the hydrogel and report the detailed porous structure and local diffusion heterogeneities not captured by the average diffusion coefficient. Conversely, bigger dextran molecules are confined and unable to freely move through the hydrogel, highlighting only the larger pore structures. These findings establish fcsSOFI as a powerful tool to characterize spatial and diffusion information of diverse macromolecules within biorelevant matrices.
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影响因子：
3.8
作者：
Dertinger T;Colyer R;Vogel R;Enderlein J;Weiss S
通讯作者：
Weiss S
影响因子：
4.4
作者：
Berezhkovskii, Alexander M.;Dagdug, Leonardo;Bezrukov, Sergey M.
通讯作者：
Bezrukov, Sergey M.
影响因子：
2.8
作者：
Curtin, Alexandra E.;Skinner, Ryan;Sanders, Aric W.
通讯作者：
Sanders, Aric W.
影响因子：
3.3
作者：
Dutta, Chayan;Bishop, Logan D. C.;Landes, Christy F.
通讯作者：
Landes, Christy F.
DOI：
10.1073/pnas.0907866106
发表时间：
2009-12-29
影响因子：
11.1
作者：
Dertinger, T.;Colyer, R.;Enderlein, J.
通讯作者：
Enderlein, J.