Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa.
Lamin-like analogues in plants: the characterization of NMCP1 in Allium cepa.
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DOI：
10.1093/jxb/ert020
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发表时间：
2013-04
影响因子：
6.9
通讯作者：
Moreno Díaz de la Espina S
中科院分区：
文献类型：
--
作者：
Ciska M;Masuda K;Moreno Díaz de la Espina S
植物的核骨架包含一个外围的纤层（也称为纤层），即使纤层蛋白在植物中不存在，它们的作用仍然在植物核中完成。植物核纤层蛋白类似物的鉴定是植物生物学中最有趣的课题之一。核纤层蛋白（Nuclear matrix constituent protein，NMCP）家族的成员具有典型的核纤层蛋白三联结构，是在植物中发挥核纤层蛋白功能的理想候选者。本文根据NMCP家族76个同源物的系统发育关系、序列相似性和保守区的分布，对NMCP家族进行了生物信息学分析和分类。此外，NMCP 1在单子叶植物洋葱其特征在于其序列和结构，生化特性，亚核分布和其在整个根中的表达的变化进行了鉴定。结果表明，这些蛋白质表现出许多相似的核纤层蛋白（结构组织，保守区域，亚核分布，和溶解度），他们可能履行核纤层蛋白在植物中的功能。这些发现大大推进了对植物叶片和核骨架结构蛋白的理解，并为进一步研究形成这些结构的蛋白质网络提供了基础。
The nucleoskeleton of plants contains a peripheral lamina (also called plamina) and, even though lamins are absent in plants, their roles are still fulfilled in plant nuclei. One of the most intriguing topics in plant biology concerns the identity of lamin protein analogues in plants. Good candidates to play lamin functions in plants are the members of the NMCP (nuclear matrix constituent protein) family, which exhibit the typical tripartite structure of lamins. This paper describes a bioinformatics analysis and classification of the NMCP family based on phylogenetic relationships, sequence similarity and the distribution of conserved regions in 76 homologues. In addition, NMCP1 in the monocot Allium cepa characterized by its sequence and structure, biochemical properties, and subnuclear distribution and alterations in its expression throughout the root were identified. The results demonstrate that these proteins exhibit many similarities to lamins (structural organization, conserved regions, subnuclear distribution, and solubility) and that they may fulfil the functions of lamins in plants. These findings significantly advance understanding of the structural proteins of the plant lamina and nucleoskeleton and provide a basis for further investigation of the protein networks forming these structures.
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影响因子：
5.6
作者：
Kapinos, Larisa E.;Schumacher, Jens;Herrmann, Harald
通讯作者：
Herrmann, Harald
DOI：
10.1083/jcb.105.1.577
发表时间：
1987-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lehner CF;Stick R;Eppenberger HM;Nigg EA
通讯作者：
Nigg EA
影响因子：
3.9
作者：
Goldberg, Martin W.;Fiserova, Jindriska;Stick, Reimer
通讯作者：
Stick, Reimer
影响因子：
5.8
作者：
Delorenzi, M;Speed, T
通讯作者：
Speed, T
影响因子：
1.6
作者：
DELAESPINA, SMD;BARTHELLEMY, I;CEREZUELA, MA
通讯作者：
CEREZUELA, MA