Comparison of cartilage histopathology assessment systems on human knee joints at all stages of osteoarthritis development.
Comparison of cartilage histopathology assessment systems on human knee joints at all stages of osteoarthritis development.
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DOI：
10.1016/j.joca.2011.12.018
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发表时间：
2012-06
影响因子：
7
通讯作者：
Lotz, M. K.
中科院分区：
文献类型：
--
作者：
Pauli, C.;Whiteside, R.;Heras, F. L.;Nesic, D.;Koziol, J.;Grogan, S. P.;Matyas, J.;Pritzker, K. P. H.;D'Lima, D. D.;Lotz, M. K.
为了比较Mankin和OARSI的软骨组织病理学评估系统，使用了大量来自成人年龄谱的代表所有水平软骨退化的人关节软骨。人膝关节(n=125，供体65个，年龄23~92岁)取自组织库。所有软骨表面均进行大体分级。取代表股骨髁、胫骨平台和膝盖骨整个中心区的骨软骨板进行组织学和藏红素O-固绿染色。5名观察者扫描了代表正常组织、老化组织和骨关节炎组织的幻灯片，并对电子图像进行了在线评分。对观察者间和观察者内的可变性、重复性和可靠性进行统计分析。Mankin系统的五个观察员之间的观察者间变异性显示出与OARSI系统相似的良好的类内系数(ICC&>0.81)(ICC&>0.78)。五位读者中有三位的重复评分显示出非常好的一致性(ICC>；0.94)。从Spearman的ROH值来看，这两个系统在五个读者中的四个中都显示出高度的重复性。对于Mankin系统，病变深度代表的表面是所有读者都表现出极好一致性的参数。其他参数，如细胞密度、藏红花O染色强度和潮汐标记，读者之间存在较大分歧。两种评分系统都是可靠的，但对于病变严重程度的评估来说，似乎过于复杂和耗时，这是标准化评分系统确定的主要参数。为了快速和可重复地评估软骨退化的严重程度，我们建议开发一种简化的损伤体积系统。
To compare the MANKIN and OARSI cartilage histopathology assessment systems using human articular cartilage from a large number of donors across the adult age spectrum representing all levels of cartilage degradation. Human knees (n=125 from 65 donors; age range 23–92) were obtained from tissue banks. All cartilage surfaces were macroscopically graded. Osteochondral slabs representing the entire central regions of both femoral condyles, tibial plateaus, and the patella were processed for histology and Safranin O – Fast Green staining. Slides representing normal, aged, and OA tissue were scanned and electronic images were scored online by five observers. Statistical analysis was performed for inter- and intra-observer variability, reproducibility and reliability. The inter-observer variability among five observers for the MANKIN system showed a similar good intra-class coefficient (ICC >0.81) as for the OARSI system (ICC >0.78). Repeat scoring by three of the five readers showed very good agreement (ICC >0.94). Both systems showed a high reproducibility among four of the five readers as indicated by the Spearman’s rho value. For the MANKIN system, the surface represented by lesion depth was the parameter where all readers showed an excellent agreement. Other parameters such as cellularity, Safranin O staining intensity and tidemark had greater inter-reader disagreement. Both scoring systems were reliable but appeared too complex and time consuming for assessment of lesion severity, the major parameter determined in standardized scoring systems. To rapidly and reproducibly assess severity of cartilage degradation, we propose to develop a simplified system for lesion volume.
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影响因子：
4.9
作者：
Kuroki, Hiroshi;Nakagawa, Yasuaki;Mori, Koji;Ohba, Mao;Suzuki, Takashi;Mizuno, Yasuyuki;Ando, Keiji;Takenaka, Makoto;Ikeuchi, Ken;Nakamura, Takashi
通讯作者：
Nakamura, Takashi
影响因子：
7
作者：
Irlenbusch, U.;Schaeller, Th.
通讯作者：
Schaeller, Th.
影响因子：
27.4
作者：
Ostergaard, K;Andersen, CB;Salter, DM
通讯作者：
Salter, DM
影响因子：
7
作者：
Gelse, K;Söder, S;Aigner, T
通讯作者：
Aigner, T
影响因子：
2
作者：
Barley, R. D. C.;Bagnall, K. M.;Jomha, N. M.
通讯作者：
Jomha, N. M.