A synergistic approach to the design, fabrication and evaluation of 3D printed micro and nano featured scaffolds for vascularized bone tissue repair.
A synergistic approach to the design, fabrication and evaluation of 3D printed micro and nano featured scaffolds for vascularized bone tissue repair.
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DOI：
10.1088/0957-4484/27/6/064001
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发表时间：
2016-02-12
期刊：
影响因子：
3.5
通讯作者：
Zhang LG
中科院分区：
文献类型：
--
作者：
Holmes B;Bulusu K;Plesniak M;Zhang LG
3D生物打印已经开始在推进功能性组织/器官替代品的发展方面显示出巨大的前景。然而，要实现3D生物打印组织用于临床的真正潜力，需要制造互连且有效的血管网络。解决这一挑战至关重要，因为人体组织依赖足够的血管网络来将氧气、营养物质、其他化学物质、生物因子和废物输送进出组织。在这里，我们成功地设计和打印了一系列新型3D骨支架，这些支架具有骨形成支持结构和高度互连的3D微血管模拟通道，用于有效和增强的成骨骨再生以及血管细胞生长。使用化学功能化过程，我们将我们的样品与纳米羟基磷灰石（nHA）结合，用于创建用于血管化骨生长的新型微米和纳米特征装置。我们评估了我们的支架与机械测试，流体力学测量和体外人间充质干细胞（hMSC）的粘附（4小时），增殖（1，3和5天）和成骨分化（1，2和3周）。这些测试证实了骨样的物理性质和血管样的流动曲线，以及证明增强的hMSC粘附，增殖和成骨分化。使用人脐静脉内皮细胞的其他体外实验也证明了微纳米特征支架上血管细胞生长、迁移和组织化的改善。
3D bioprinting has begun to show great promise in advancing the development of functional tissue/organ replacements. However, to realize the true potential of 3D bioprinted tissues for clinical use requires the fabrication of an interconnected and effective vascular network. Solving this challenge is critical, as human tissue relies on an adequate network of blood vessels to transport oxygen, nutrients, other chemicals, biological factors and waste, in and out of the tissue. Here, we have successfully designed and printed a series of novel 3D bone scaffolds with both bone formation supporting structures and highly interconnected 3D microvascular mimicking channels, for efficient and enhanced osteogenic bone regeneration as well as vascular cell growth. Using a chemical functionalization process, we have conjugated our samples with nano hydroxyapatite (nHA), for the creation of novel micro and nano featured devices for vascularized bone growth. We evaluated our scaffolds with mechanical testing, hydrodynamic measurements and in vitro human mesenchymal stem cell (hMSC) adhesion (4 h), proliferation (1, 3 and 5 d) and osteogenic differentiation (1, 2 and 3 weeks). These tests confirmed bone-like physical properties and vascular-like flow profiles, as well as demonstrated enhanced hMSC adhesion, proliferation and osteogenic differentiation. Additional in vitro experiments with human umbilical vein endothelial cells also demonstrated improved vascular cell growth, migration and organization on micro-nano featured scaffolds.
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影响因子：
9.7
作者：
Andukuri, Adinarayana;Kushwaha, Meenakshi;Tambralli, Ajay;Anderson, Joel M.;Dean, Derrick R.;Berry, Joel L.;Sohn, Young Doug;Yoon, Young-Sup;Brott, Brigitta C.;Jun, Ho-Wook
通讯作者：
Jun, Ho-Wook
影响因子：
4
作者：
Grellier, Maritie;Ferreira-Tojais, Nancy;Amedee, Joelle
通讯作者：
Amedee, Joelle
影响因子：
1.9
作者：
Celik, M;Tuncer, S;Kesim, SN
通讯作者：
Kesim, SN
影响因子：
3.9
作者：
Aishwarya, S.;Mahalakshmi, S.;Sehgal, Praveen Kumar
通讯作者：
Sehgal, Praveen Kumar
DOI：
10.1002/jbm.b.10485
发表时间：
2003-02-15
影响因子：
3.4
作者：
Cooke, MN;Fisher, JP;Mikos, AG
通讯作者：
Mikos, AG