Oligoarray comparative genomic hybridization-mediated mapping of suppressor mutations generated in a deletion-biased mutagenesis screen.
Oligoarray comparative genomic hybridization-mediated mapping of suppressor mutations generated in a deletion-biased mutagenesis screen.
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DOI：
10.1534/g3.112.002238
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发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
Baillie DL
中科院分区：
文献类型：
--
作者：
Jones MR;Rose AM;Baillie DL
抑制筛选是一种非常宝贵的方法，用于识别模式生物秀丽隐杆线虫基因之间的新的遗传相互作用。然而，传统上，这种方法在分子水平上定位突变的过程费力而漫长。使用已知产生小缺失的诱变剂，再加上寡核苷酸阵列比较基因组杂交（aCGH），我们已经确定了两个基因的突变，抑制与必需受体酪氨酸激酶rol-3突变相关的致死性。首先，我们发现Bicaudal-C直系同源物bcc-1的缺失抑制了rol-3相关的致死性。第二，我们确定了几个重复，也抑制了rol-3相关的致死性。我们确定srap-1的过度表达，一个存在于这些重复中的单一基因，介导了这种抑制。这项研究表明，删除偏置诱变筛选与aCGH表征相结合，用于快速鉴定新的抑制突变的适用性。除了检测小的缺失，这种方法是适合于确定拷贝数抑制突变，一类抑制不容易使用替代方法的特点。
Suppressor screens are an invaluable method for identifying novel genetic interactions between genes in the model organism Caenorhabditis elegans. However, traditionally this approach has suffered from the laborious and protracted process of mapping mutations at the molecular level. Using a mutagen known to generate small deletions, coupled with oligoarray comparative genomic hybridization (aCGH), we have identified mutations in two genes that suppress the lethality associated with a mutation of the essential receptor tyrosine kinase rol-3. First, we find that deletion of the Bicaudal-C ortholog, bcc-1, suppresses rol-3–associated lethality. Second, we identify several duplications that also suppress rol-3–associated lethality. We establish that overexpression of srap-1, a single gene present in these duplications, mediates the suppression. This study demonstrates the suitability of deletion-biased mutagenesis screening in combination with aCGH characterization for the rapid identification of novel suppressor mutations. In addition to detecting small deletions, this approach is suitable for identifying copy number suppressor mutations, a class of suppressor not easily characterized using alternative approaches.
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影响因子：
9.2
作者：
Lipinski, Kendra J.;Farslow, James C.;Fitzpatrick, Kelly A.;Lynch, Michael;Katju, Vaishali;Bergthorsson, Ulfar
通讯作者：
Bergthorsson, Ulfar
影响因子：
3.5
作者：
Kage-Nakadai E;Kobuna H;Funatsu O;Otori M;Gengyo-Ando K;Yoshina S;Hori S;Mitani S
通讯作者：
Mitani S
DOI：
10.1006/bbrc.2000.2260
发表时间：
2000-03-05
影响因子：
3.1
作者：
Gengyo-Ando, K;Mitani, S
通讯作者：
Mitani, S
影响因子：
11.8
作者：
Eckmann, CR;Kraemer, B;Kimble, J
通讯作者：
Kimble, J
影响因子：
4.4
作者：
Maydan JS;Lorch A;Edgley ML;Flibotte S;Moerman DG
通讯作者：
Moerman DG