Effects of long-term treatment with 17 beta-estradiol and medroxyprogesterone acetate on water maze performance in middle aged female rats.
Effects of long-term treatment with 17 beta-estradiol and medroxyprogesterone acetate on water maze performance in middle aged female rats.
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DOI：
10.1016/j.yhbeh.2010.03.018
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发表时间：
2010-07
影响因子：
3.5
通讯作者：
Juraska, Janice M.
中科院分区：
文献类型：
--
作者：
Lowry, Nioka C.;Pardon, Laura P.;Yates, Melissa A.;Juraska, Janice M.
关键词：
尽管之前的研究表明激素替代疗法有益于更年期女性的记忆力，但最近的几项研究表明治疗要么有害，要么没有影响。这些不一致强调需要在动物模型中评估卵巢激素在预防与年龄相关的认知能力下降方面的作用。本研究调查了衰老过程中长期激素治疗对莫里斯水迷宫的影响。雌性长伊文思罩帽大鼠在中年（12-13个月）被切除卵巢，并立即分为五组之一：无替代、仅慢性17β-雌二醇、慢性17β-雌二醇和黄体酮、慢性17β-雌二醇和醋酸甲羟孕酮（MPA）或仅环状17β-雌二醇。以慢性或周期性（4 天中的 3 天）方式在饮用水中施用 17 β-雌二醇。黄体酮和 MPA 通过皮下颗粒给药。经过六个月的激素治疗后，动物在莫里斯水迷宫上进行了测试。动物每天进行四次试验，持续四天，在测试的最后一天后，一部分动物完成了探索试验。在四天的测试中，接受 17 β-雌二醇联合 MPA 治疗的大鼠的表现明显低于接受激素替代治疗的所有其他组。此外，在测试的最后一天，长期17β-雌二醇给药比循环给药且无替代疗法更有益。因此，与其他激素治疗组相比，长期 17 β-雌二醇治疗与 MPA 组合会导致空间莫里斯水迷宫的表现受损。
Although previous research has indicated that hormone replacement therapy benefits memory in menopausal women, several recent studies have shown either detrimental or no effects of treatment. These inconsistencies emphasize the need to evaluate the role of ovarian hormones in protecting against age-related cognitive decline in an animal model. The present study investigated the effects of long term hormone treatment during aging on the Morris water maze. Female Long Evans hooded rats were ovariectomized at middle age (12–13 months) and were immediately placed in one of five groups: no replacement, chronic 17 β-estradiol only, chronic 17 β-estradiol and progesterone, chronic 17 β-estradiol and medroxyprogesterone acetate (MPA), or cyclic 17 β-estradiol only. 17 β-estradiol was administered in the drinking water in either a chronic or cyclic (3 out of 4 days) fashion. Progesterone and MPA were administered via subcutaneous pellets. Following six months of hormone treatment, animals were tested on the Morris water maze. Animals performed four trials a day for four days and after the final day of testing a subset of animals completed a probe trial. Across four days of testing, rats receiving 17 β-estradiol in combination with MPA performed significantly worse than all other groups receiving hormone replacement. In addition on the last day of testing, chronic 17 β-estradiol administration was more beneficial than cyclic administration and no replacement. Thus compared to other hormone treated groups, long term 17 β-estradiol treatment in combination with MPA results in impaired performance on the spatial Morris water maze.
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DOI：
10.1002/neu.20293
发表时间：
2006-08-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Ciriza, Iratxe;Carrero, Paloma;Garcia-Segura, Luis M.
通讯作者：
Garcia-Segura, Luis M.
影响因子：
7.2
作者：
KAMPEN, DL;SHERWIN, BB
通讯作者：
SHERWIN, BB
影响因子：
2.3
作者：
Acheson, SK;Ross, EL;Swartzwelder, HS
通讯作者：
Swartzwelder, HS
影响因子：
3.5
作者：
Duff, SJ;Hampson, E
通讯作者：
Hampson, E
影响因子：
3.2
作者：
Daniel, J. M.
通讯作者：
Daniel, J. M.