Housekeeping and tissue-specific genes in mouse tissues.
Housekeeping and tissue-specific genes in mouse tissues.
复制标题
DOI：
10.1186/1471-2164-8-127
复制
发表时间：
2007-05-22
期刊：
影响因子：
4.4
通讯作者：
St-Amand J
中科院分区：
文献类型：
--
作者：
Kouadjo KE;Nishida Y;Cadrin-Girard JF;Yoshioka M;St-Amand J
本研究旨在通过使用基因表达系列分析（SAGE）策略来表征15个小鼠组织中的管家基因和组织特异性基因，该策略指示与标签匹配的每个转录本的相对表达水平。在这里，我们确定了持续表达的管家基因，如真核翻译延伸因子2，它在所有组织中表达，表达水平无显著差异。而且，这些基因中的大多数不受实验条件的调控，如类固醇激素、肾上腺切除术和性腺切除术。此外，我们报告先前假定的管家基因，如肽基脯氨酰顺反异构酶A，甘油醛-3-磷酸脱氢酶和β-肌动蛋白，这些基因在所有组织中表达，但其表达水平有显着差异。我们还从公共数据库中确定了在15种组织和其他组织中唯一检测到的基因。当比较几种组织中基因的表达水平时，这些鉴定的管家基因可以代表RT-PCR和北方印迹的适当对照。结果表明，一些组织特异性基因在睾丸和垂体中高表达。此外，在肝、肺和骨中表达的组织特异性基因的主要功能是细胞防御，而参与细胞结构功能的几种角蛋白仅在皮肤和阴道中检测到。该研究的结果可用于例如通过使用组织特异性基因的启动子来靶向组织进行药剂递送。本研究为进一步研究这些组织的生理和病理学奠定了基础。
This study aims to characterize the housekeeping and tissue-specific genes in 15 mouse tissues by using the serial analysis of gene expression (SAGE) strategy which indicates the relative level of expression for each transcript matched to the tag. Here, we identified constantly expressed housekeeping genes, such as eukaryotic translation elongation factor 2, which is expressed in all tissues without significant difference in expression levels. Moreover, most of these genes were not regulated by experimental conditions such as steroid hormones, adrenalectomy and gonadectomy. In addition, we report previously postulated housekeeping genes such as peptidyl-prolyl cis-trans isomerase A, glyceraldehyde-3-phosphate dehydrogenase and beta-actin, which are expressed in all the tissues, but with significant difference in their expression levels. We have also identified genes uniquely detected in each of the 15 tissues and other tissues from public databases. These identified housekeeping genes could represent appropriate controls for RT-PCR and northern blot when comparing the expression levels of genes in several tissues. The results reveal several tissue-specific genes highly expressed in testis and pituitary gland. Furthermore, the main function of tissue-specific genes expressed in liver, lung and bone is the cell defence, whereas several keratins involved in cell structure function are exclusively detected in skin and vagina. The results from this study can be used for example to target a tissue for agent delivering by using the promoter of tissue-specific genes. Moreover, this study could be used as basis for further researches on physiology and pathology of these tissues.
登录
查看更多内容
人骨髓中嗜中性粒细胞多形核白细胞的发展。
影响因子：
15.3
作者：
Bainton, D F;Ullyot, J L;Farquhar, M G
通讯作者：
Farquhar, M G
影响因子：
14.9
作者：
Dinel S;Bolduc C;Belleau P;Boivin A;Yoshioka M;Calvo E;Piedboeuf B;Snyder EE;Labrie F;St-Amand J
通讯作者：
St-Amand J
DOI：
10.1073/pnas.0507480102
发表时间：
2005-12-27
影响因子：
11.1
作者：
Boutrel, B;Kenny, PJ;de Lecea, L
通讯作者：
de Lecea, L
影响因子：
56.9
作者：
Fremeau, RT;Kam, K;Edwards, RH
通讯作者：
Edwards, RH
影响因子：
3
作者：
Ganapathi M;Srivastava P;Das Sutar SK;Kumar K;Dasgupta D;Pal Singh G;Brahmachari V;Brahmachari SK
通讯作者：
Brahmachari SK