Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface.
Kinesin-5/Eg5 is important for transport of CARTS from the trans-Golgi network to the cell surface.
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DOI：
10.1083/jcb.201303163
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发表时间：
2013-07-22
期刊：
影响因子：
--
通讯作者：
Malhotra V
中科院分区：
文献类型：
--
作者：
Wakana Y;Villeneuve J;van Galen J;Cruz-Garcia D;Tagaya M;Malhotra V
驱动蛋白-5马达Eg 5在将一类特定的运输载体（CARTS）从trans-Golgi网络运输到细胞表面中具有新颖的非有丝分裂作用。在这里，我们报告的驱动蛋白-5马达Klp 61 F，这是已知的双极纺锤体形成在有丝分裂中的作用，是必需的蛋白质运输从高尔基复合体的细胞表面在果蝇S2细胞。在HeLa细胞中，破坏其哺乳动物直系同源物Eg 5的功能抑制了一种称为胰腺癌上调因子（PAUF）的蛋白质的分泌，但令人惊讶的是，没有将水泡性口炎病毒G蛋白（VSV-G）运输到细胞表面。我们之前曾报道过PAUF通过称为CARTS的特定载体从跨高尔基体网络（TGN）运输到细胞表面，该载体不包括VSV-G。Eg 5功能的抑制并不影响CARTS的生物发生;然而，它们的迁移被延迟，并在高尔基复合体附近积累。总而言之，我们的研究结果揭示了一个令人惊讶的新的作用Eg 5在非有丝分裂细胞中的运输的特定载体，CARTS，从TGN到细胞表面的便利。
The kinesin-5 motor Eg5 has a novel non-mitotic role in the transport of a specific class of transport carriers (CARTS) from the trans-Golgi network to the cell surface. Here we report that the kinesin-5 motor Klp61F, which is known for its role in bipolar spindle formation in mitosis, is required for protein transport from the Golgi complex to the cell surface in Drosophila S2 cells. Disrupting the function of its mammalian orthologue, Eg5, in HeLa cells inhibited secretion of a protein called pancreatic adenocarcinoma up-regulated factor (PAUF) but, surprisingly, not the trafficking of vesicular stomatitis virus G protein (VSV-G) to the cell surface. We have previously reported that PAUF is transported from the trans-Golgi network (TGN) to the cell surface in specific carriers called CARTS that exclude VSV-G. Inhibition of Eg5 function did not affect the biogenesis of CARTS; however, their migration was delayed and they accumulated near the Golgi complex. Altogether, our findings reveal a surprising new role of Eg5 in nonmitotic cells in the facilitation of the transport of specific carriers, CARTS, from the TGN to the cell surface.
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
11.8
作者：
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影响因子：
7.8
作者：
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通讯作者：
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7.8
作者：
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影响因子：
9.2
作者：
Kwok, BH;Yang, JG;Kapoor, TM
通讯作者：
Kapoor, TM