Transcriptomes and neurotransmitter profiles of classes of gustatory and somatosensory neurons in the geniculate ganglion.
Transcriptomes and neurotransmitter profiles of classes of gustatory and somatosensory neurons in the geniculate ganglion.
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DOI：
10.1038/s41467-017-01095-1
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发表时间：
2017-10-02
影响因子：
16.6
通讯作者：
Chaudhari N
中科院分区：
文献类型：
--
作者：
Dvoryanchikov G;Hernandez D;Roebber JK;Hill DL;Roper SD;Chaudhari N
味蕾受神经元支配，神经元的细胞体位于颅内感觉神经节中。由于缺乏定义其分子身份和类别的标记，对这些神经元的功能特性和连接性的研究受到阻碍。小鼠膝状神经节包含支配舌和腭味蕾的化学感觉神经元和支配耳廓的体感神经元。在这里，我们报告了膝状神经节神经元的单细胞 RNA 测序。使用无偏转录组分析，我们显示了两个主要簇之间的明显分离，通过顺行标记，这两个主要簇对应于味觉和体感神经元。在味觉神经元中，存在三个亚群，每个亚群都有自己的转录因子和神经递质反应谱。最小的亚簇表达味觉和机械感觉相关基因，表明感觉神经元是一种新型类型。我们确定了几个标记来帮助剖析味觉神经元之间的功能差异，并解决有关目标相互作用和味觉编码的问题。由于缺乏分子标记，味觉神经通路的表征受到影响。在此，作者报告了单细胞 RNA 测序和无偏转录组分析，揭示了味觉神经元和体感神经元以及具有独特分子和功能特征的味觉神经元亚群之间的主要区别。
Taste buds are innervated by neurons whose cell bodies reside in cranial sensory ganglia. Studies on the functional properties and connectivity of these neurons are hindered by the lack of markers to define their molecular identities and classes. The mouse geniculate ganglion contains chemosensory neurons innervating lingual and palatal taste buds and somatosensory neurons innervating the pinna. Here, we report single cell RNA sequencing of geniculate ganglion neurons. Using unbiased transcriptome analyses, we show a pronounced separation between two major clusters which, by anterograde labeling, correspond to gustatory and somatosensory neurons. Among the gustatory neurons, three subclusters are present, each with its own complement of transcription factors and neurotransmitter response profiles. The smallest subcluster expresses both gustatory- and mechanosensory-related genes, suggesting a novel type of sensory neuron. We identify several markers to help dissect the functional distinctions among gustatory neurons and address questions regarding target interactions and taste coding. Characterization of gustatory neural pathways has suffered due to a lack of molecular markers. Here, the authors report single cell RNA sequencing and unbiased transcriptome analyses to reveal major distinctions between gustatory and somatosensory neurons and subclusters of gustatory neurons with unique molecular and functional profiles.
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DOI：
10.1523/jneurosci.5559-10.2011
发表时间：
2011-04-13
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
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