A standardized workflow for submitting data to the Minimum Information about a Biosynthetic Gene cluster (MIBiG) repository: prospects for research-based educational experiences.
A standardized workflow for submitting data to the Minimum Information about a Biosynthetic Gene cluster (MIBiG) repository: prospects for research-based educational experiences.
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DOI：
10.1186/s40793-018-0318-y
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发表时间：
2018
影响因子：
--
通讯作者：
Medema MH
中科院分区：
文献类型：
--
作者：
Epstein SC;Charkoudian LK;Medema MH
微生物利用复杂的酶途径生物合成结构复杂和药理学相关的分子。这些途径是由基因簇编码的，在多种生物体中都有发现。通过利用用户提交的数据将科学发现转化为可计算分析的格式，关于生物合成基因簇存储库的最小信息促进了这些基因簇及其分子产物实验数据的标准化和集中存储。这加速了将基因与化学结构连接起来的过程，在环境多样性的背景下理解生物合成基因簇，并执行合成基因簇的计算机辅助设计。在这里，我们提供了一个标准操作程序，Excel模板，教程视频和相关综述文献的集合，以支持科学家们向MiBIG提交数据。此外，我们还提供了将基因簇注释项目集成到课堂环境中的工具，包括工作流程和评估材料。本文的在线版本（10.1186/s40793-018-0318-y）包含补充材料，授权用户可以使用。
Microorganisms utilize complex enzymatic pathways to biosynthesize structurally complex and pharmacologically relevant molecules. These pathways are encoded by gene clusters and are found in a diverse set of organisms. The Minimum Information about a Biosynthetic Gene cluster repository facilitates standardized and centralized storage of experimental data on these gene clusters and their molecular products, by utilizing user-submitted data to translate scientific discoveries into a format that can be analyzed computationally. This accelerates the processes of connecting genes to chemical structures, understanding biosynthetic gene clusters in the context of environmental diversity, and performing computer-assisted design of synthetic gene clusters. Here, we present a Standard Operating Procedure, Excel templates, a tutorial video, and a collection of relevant review literature to support scientists in their efforts to submit data into MiBIG. Further, we provide tools to integrate gene cluster annotation projects into the classroom environment, including workflows and assessment materials. The online version of this article (10.1186/s40793-018-0318-y) contains supplementary material, which is available to authorized users.
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发表时间：
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期刊：
CBE life sciences education
影响因子：
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作者：
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