Injectable, porous, and cell-responsive gelatin cryogels.
Injectable, porous, and cell-responsive gelatin cryogels.
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DOI：
10.1016/j.biomaterials.2013.11.044
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发表时间：
2014-03
期刊：
影响因子：
14
通讯作者：
Mooney, David J.
中科院分区：
文献类型：
--
作者：
Koshy, Sandeep T.;Ferrante, Thomas C.;Lewin, Sarah A.;Mooney, David J.
与外科植入物相关的并发症可能会阻碍基于生物材料的治疗的性能，从而促使开发允许微创引入宿主的材料系统。在这项研究中，我们创造了细胞粘附和可降解的明胶支架，可以通过传统的针头注射，同时保持预定义的几何形状和结构。这些支架支持细胞在体外的附着、增殖和存活，并且可以被重组基质金属蛋白酶-2和-9降解。当皮下注射到小鼠体内时，预制明胶冷冻凝胶迅速恢复其原始形状，并且在注射后仅引起轻微的宿主反应。从明胶冻凝胶中控制释放粒细胞-巨噬细胞集落刺激因子导致免疫细胞完全浸润支架，并促进基质金属蛋白酶的产生，导致细胞介导的冻凝胶基质降解。这些研究结果表明，明胶冷冻凝胶可以作为一个细胞响应平台的生物材料为基础的治疗。
The performance of biomaterials-based therapies can be hindered by complications associated with surgical implant, motivating the development of materials systems that allow minimally invasive introduction into the host. In this study, we created cell-adhesive and degradable gelatin scaffolds that could be injected through a conventional needle while maintaining a predefined geometry and architecture. These scaffolds supported attachment, proliferation, and survival of cells in vitro and could be degraded by recombinant matrix metalloproteinase-2 and -9. Prefabricated gelatin cryogels rapidly reassumed their original shape when injected subcutaneously into mice and elicited only a minor host response following injection. Controlled release of granulocyte-macrophage colony-stimulating factor from gelatin cryogels resulted in complete infiltration of the scaffold by immune cells and promoted matrix metalloproteinase production leading to cell-mediated degradation of the cryogel matrix. These findings suggest that gelatin cryogels could serve as a cell-responsive platform for biomaterial-based therapy.
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影响因子：
14
作者：
Nichol, Jason W.;Koshy, Sandeep T.;Bae, Hojae;Hwang, Chang M.;Yamanlar, Seda;Khademhosseini, Ali
通讯作者：
Khademhosseini, Ali
影响因子：
14
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
14
作者：
Lin, Ruei-Zeng;Chen, Ying-Chieh;Moreno-Luna, Rafael;Khademhosseini, Ali;Melero-Martin, Juan M.
通讯作者：
Melero-Martin, Juan M.
影响因子：
7
作者：
Bae, Ki Hyun;Wang, Li-Shan;Kurisawa, Motoichi
通讯作者：
Kurisawa, Motoichi
影响因子：
4.1
作者：
Mollinedo, F;Nakajima, M;Fabra, A
通讯作者：
Fabra, A