Energy metabolism and T-cell-mediated cytolysis. I. Synergism between inhibitors of respiration and glycolysis.
Energy metabolism and T-cell-mediated cytolysis. I. Synergism between inhibitors of respiration and glycolysis.
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能量代谢和T细胞介导的细胞解析。 I.呼吸和糖酵解抑制剂之间的协同作用。
DOI：
10.1084/jem.146.3.698
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发表时间：
1977-09-01
期刊：
影响因子：
--
通讯作者：
Koch CJ
中科院分区：
文献类型：
--
作者：
MacDonald HR;Koch CJ
已经研究了T细胞介导的细胞溶解的能量需求。在混合白细胞培养物中体外产生溶细胞胸腺衍生淋巴细胞（CTL），并测定其对51 Cr标记的肥大细胞瘤靶细胞的细胞毒性。在不存在外源性葡萄糖（小于5微米）或在极端缺氧（小于0.2微米氧）的条件下，细胞溶解仅略微降低。此外，葡萄糖类似物2-脱氧-D-葡萄糖和5-硫代-D-葡萄糖以及呼吸拮抗剂叠氮化钠和2，4-二硝基苯酚在单独使用时都不是非常有效的细胞溶解抑制剂。然而，这些葡萄糖类似物在无氧条件下抑制细胞溶解非常有效，呼吸拮抗剂在无葡萄糖条件下抑制细胞溶解的程度要大得多。此外，当糖酵解和呼吸抑制剂组合时，观察到协同效应。总之，这些结果表明T细胞介导的细胞溶解是一种能量依赖性过程，其可以由氧化或糖酵解能量途径支持。
The energy requirements for T-cell-mediated cytolysis have been investigated. Cytolytic thymus-derived lymphocytes (CTL) were generated in vitro in mixed leukocyte cultures and assayed for cytotoxicity on 51Cr-labeled mastocytoma target cells. Cytolysis was only slightly reduced in the absence of exogenous glucose (less than 5 micrometer) or under conditions of extreme hypoxia (less than 0.2 micrometer oxygen). Furthermore, neither the glucose analogues 2-deoxy-D-glucose and 5-thio- D-glucose nor the respiratory antagonists sodium azide and 2,4- dinitrophenol were very effective inhibitors of cytolysis when used individually. However, these glucose analogues were highly effective in inhibiting cytolysis in the absence of oxygen, and the respiratory antagonists inhibited cytolysis to a much greater extent in the absence of glucose. In addition, synergistic effects were observed when the glycolytic and respiratory inhibitors were combined. Taken together, these results indicate that T-cell-mediated cytolysis is an energy- dependent process which can be supported by either oxidative or glycolytic energy pathways.
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影响因子：
3.4
作者：
KOCH, CJ;PAINTER, RB
通讯作者：
PAINTER, RB
影响因子：
3.7
作者：
MACDONALD, HR;BONNARD, GD
通讯作者：
BONNARD, GD
影响因子：
3.7
作者：
ROOS, D;LOOS, JA
通讯作者：
LOOS, JA
DOI：
10.1182/blood.v30.6.691.691
发表时间：
1967-01-01
期刊：
BLOOD-THE JOURNAL OF HEMATOLOGY
影响因子：
--
作者：
PACHMAN, LM
通讯作者：
PACHMAN, LM
影响因子：
4.3
作者：
STROM, TB;GAROVOY, MR;CARPENTER, CB
通讯作者：
CARPENTER, CB