Changes in Morphology and Presence of Pinopodes in Endometrial Cells during the Luteal Phase in Women with Infertility Problems: A Pilot Study.
Changes in Morphology and Presence of Pinopodes in Endometrial Cells during the Luteal Phase in Women with Infertility Problems: A Pilot Study.
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DOI：
10.3390/medicina54050069
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发表时间：
2018-10-10
期刊：
影响因子：
--
通讯作者：
Arend A
中科院分区：
文献类型：
--
作者：
Aunapuu M;Kibur P;Järveots T;Arend A
目的：探讨不孕症患者子宫内膜上皮细胞的形态学变化。材料与方法：子宫内膜活检获得了10名妇女谁经历了几个不成功的体外受精（IVF）程序。在自然月经周期的LH+6至LH +10的黄体生成素高峰期进行子宫内膜活检。将每个样品分成三部分，分别进行组织学、透射（TEM）和扫描电子显微镜（SEM）研究。结果如下：组织学研究表明，在一个病人的上皮细胞的顶端部分的显着变化，在四个病人，腺体成熟度低，不匹配的周期日，因此，一个相位滞后发展。通过透射电镜检查，我们确定了3例患者的分泌细胞和纤毛细胞的变化（微绒毛数量减少或缺失，纤毛细胞中纤毛不规则）。扫描电镜检查发现5例患者的pinopodes：三个样本含有完全发育的pinopodes较大，完全光滑，只有一些皱纹;一个样本含有退化的小pinopodes，皱纹的表面;和一个样本既发达和退化pinopodes。结论：总之，我们的研究表明，IVF结局不佳的患者的子宫内膜要么在形态学上有显著变化，要么子宫内膜成熟受到抑制，往往出现相位滞后。我们的研究表明，子宫内膜胞饮突在整个黄体中期直至LH+10天都存在。
Objective: To investigate morphological changes in the endometrial epithelial cells of patients with infertility problems. Materials and methods: Endometrial biopsies were obtained from 10 women who have undergone several unsuccessful in vitro fertilisation (IVF) procedures. Endometrial biopsies were performed between luteinizing hormone surge days LH+6 to +10 of the natural menstrual cycle. Each sample was divided into three parts, which were processed for histological, transmission (TEM), and scanning electron microscopy (SEM) investigations. Results: Histological investigations demonstrated significant alterations in the apical part of epithelial cells of one patient; in four patients, the gland maturity was low, not matching the cycle day, and thus a phase lag had developed. By TEM examination, we ascertained changes in secretory and ciliated cells in three patients (decreased amount or missing microvilli, irregular cilia in ciliated cells). SEM examination found pinopodes in five patients: three samples contained fully developed pinopodes—larger and completely smooth, with only some wrinkles; one sample contained regressing small pinopodes, with wrinkled surfaces; and one sample had both developed and regressing pinopodes. Conclusions: To conclude, our study shows that the endometrium of patients with poor IVF outcome has either significant changes in the morphology or the endometrial maturation is inhibited and a phase lag often develops. Our study shows that endometrial pinopodes are found throughout the mid-luteal phase up to day LH+10.
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影响因子：
6.7
作者：
Coutifaris, C;Myers, ER;Leppert, PC
通讯作者：
Leppert, PC
植入时的 Pinopode 评分可预测激素替代周期中冷冻胚胎移植后的成功植入
影响因子：
6.1
作者：
Jin, X. Y.;Zhao, L. J.;Zhang, S. Y.
通讯作者：
Zhang, S. Y.
影响因子：
8.2
作者：
Lopata, Alex;Bentin-Ley, Ursula;Enders, Allen
通讯作者：
Enders, Allen
影响因子：
4
作者：
Stavreus-Evers, A;Aghajanova, L;Hovatta, O
通讯作者：
Hovatta, O
影响因子：
6.1
作者：
Nikas, G
通讯作者：
Nikas, G