Preparation and culture of bone marrow-derived macrophages from mice for functional analysis.
Preparation and culture of bone marrow-derived macrophages from mice for functional analysis.
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DOI：
10.1016/j.xpro.2020.100246
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Ueki K
中科院分区：
文献类型：
--
作者：
Toda G;Yamauchi T;Kadowaki T;Ueki K
由于巨噬细胞存在多种亚型，其功能评估一直是一个备受热议的话题。本实验方案描述了从小鼠股骨和胫骨采集骨髓细胞，使其分化为骨髓来源的巨噬细胞（BMDM细胞），以及从培养物中取样的过程。本方案着重于高效制备BMDM细胞，为评估巨噬细胞的功能提供了一种方法。
有关本方案的使用和实施的完整细节，请参考……
从小鼠快速高效地采集骨髓
分化为CD11b和F4/80阳性率>90%的BMDM
培养BMDM用于RNA和蛋白质的体外功能分析
在不假定极化为M1/M2亚型的情况下对细胞进行评估
由于巨噬细胞存在多种亚型，其功能评估一直是一个备受热议的话题。本实验方案描述了从小鼠股骨和胫骨采集骨髓细胞，使其分化为骨髓来源的巨噬细胞（BMDM细胞），以及从培养物中取样的过程。本方案着重于高效制备BMDM细胞，为评估巨噬细胞的功能提供了一种方法。
The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to. Fast and efficient collection of bone marrow from mice Differentiation into BMDM that are >90% positive for CD11b and F4/80 Culture of BMDM for in vitro functional analysis of RNA and protein Cells can be assessed without assuming polarization to M1/M2 subtype The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages.
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影响因子：
--
作者：
Zhang, Xia;Goncalves, Ricardo;Mosser, David M
通讯作者：
Mosser, David M
DOI：
10.1101/pdb.prot5080
发表时间：
2008-12-01
期刊：
CSH protocols
影响因子：
--
作者：
Weischenfeldt, Joachim;Porse, Bo
通讯作者：
Porse, Bo
影响因子：
32.4
作者：
Murray, Peter J.;Allen, Judith E.;Biswas, Subhra K.;Fisher, Edward A.;Gilroy, Derek W.;Goerdt, Sergij;Gordon, Siamon;Hamilton, John A.;Ivashkiv, Lionel B.;Lawrence, Toby;Locati, Massimo;Mantovani, Alberto;Martinez, Fernando O.;Mege, Jean-Louis;Mosser, David M.;Natoli, Gioacchino;Saeij, Jeroen P.;Schultze, Joachim L.;Shirey, Kari Ann;Sica, Antonio;Suttles, Jill;Udalova, Irina;van Ginderachter, Jo A.;Vogel, Stefanie N.;Wynn, Thomas A.
通讯作者：
Wynn, Thomas A.
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.6
作者：
Lobbins, M. L. Brown;Shivakumar, B. R.;Hasty, K. A.
通讯作者：
Hasty, K. A.