Microbial shifts in the aging mouse gut.
Microbial shifts in the aging mouse gut.
复制标题
DOI：
10.1186/s40168-014-0050-9
复制
发表时间：
2014
期刊：
影响因子：
15.5
通讯作者：
Beiko RG
中科院分区：
文献类型：
--
作者：
Langille MG;Meehan CJ;Koenig JE;Dhanani AS;Rose RA;Howlett SE;Beiko RG
衰老个体的微生物组发生的变化尚不清楚，特别是考虑到虚弱与年龄的不完全相关性。对老年人的研究报告了微妙的影响，但这些结果可能会被其他经常随年龄变化的变量（如饮食和居住地）所混淆。为了在一个更可控的模型系统中测试这些关联，我们研究了雌性C57BL/6 J小鼠的年龄、虚弱和肠道微生物组之间的关系。虚弱指数是基于对小鼠31种临床恶化症状的评估得出的，它显示出与年龄近乎完美的相关性。我们观察到年龄与相应微生物组的分类组成之间存在统计学上显著的关系。与先前的人类研究一致，Rikenellaceae家族，包括Alistipes属，是中年和老年小鼠中最明显的过度代表分类群。小鼠肠道微生物组的功能也随着宿主的年龄和虚弱而变化。细菌编码的功能在老年小鼠中未被充分代表，包括钴胺素（B12）和生物素（B7）生物合成，以及与DNA修复相关的细菌SOS基因。相反，与肌肉萎缩相关的肌酸降解在老年小鼠的肠道微生物群中被过度代表，细菌编码的β-葡萄糖醛酸酶也是如此，它可以影响药物诱导的上皮细胞毒性。老年小鼠也显示单糖利用基因相对于二糖、寡糖和多糖利用基因过多，这可能对肠道稳态有实质性影响。我们已经确定了小鼠微生物组中与年龄和虚弱相关的分类和功能模式。与宿主营养和药物药理相关的功能差异以年龄依赖的方式变化，这表明基本功能的可用性和时间可能因年龄和虚弱而显着不同。未来对更大的小鼠群体的研究将旨在分离年龄、虚弱和其他因素的影响。本文的在线版本（doi:10.1186/s40168-014-0050-9）包含补充材料，可供授权用户使用。
The changes that occur in the microbiome of aging individuals are unclear, especially in light of the imperfect correlation of frailty with age. Studies in older human subjects have reported subtle effects, but these results may be confounded by other variables that often change with age such as diet and place of residence. To test these associations in a more controlled model system, we examined the relationship between age, frailty, and the gut microbiome of female C57BL/6 J mice. The frailty index, which is based on the evaluation of 31 clinical signs of deterioration in mice, showed a near-perfect correlation with age. We observed a statistically significant relationship between age and the taxonomic composition of the corresponding microbiome. Consistent with previous human studies, the Rikenellaceae family, which includes the Alistipes genus, was the most significantly overrepresented taxon within middle-aged and older mice. The functional profile of the mouse gut microbiome also varied with host age and frailty. Bacterial-encoded functions that were underrepresented in older mice included cobalamin (B12) and biotin (B7) biosynthesis, and bacterial SOS genes associated with DNA repair. Conversely, creatine degradation, associated with muscle wasting, was overrepresented within the gut microbiomes of the older mice, as were bacterial-encoded β-glucuronidases, which can influence drug-induced epithelial cell toxicity. Older mice also showed an overabundance of monosaccharide utilization genes relative to di-, oligo-, and polysaccharide utilization genes, which may have a substantial impact on gut homeostasis. We have identified taxonomic and functional patterns that correlate with age and frailty in the mouse microbiome. Differences in functions related to host nutrition and drug pharmacology vary in an age-dependent manner, suggesting that the availability and timing of essential functions may differ significantly with age and frailty. Future work with larger cohorts of mice will aim to separate the effects of age and frailty, and other factors. The online version of this article (doi:10.1186/s40168-014-0050-9) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
影响因子：
1.9
作者：
HOVE, H;NORDGAARDANDERSEN, I;MORTENSEN, PB
通讯作者：
MORTENSEN, PB
影响因子：
3
作者：
Fahlen, Annika;Engstrand, Lars;Fry, Lionel
通讯作者：
Fry, Lionel
DOI：
10.1073/pnas.1019378108
发表时间：
2011-03-29
影响因子：
11.1
作者：
Ichinohe, Takeshi;Pang, Iris K.;Iwasaki, Akiko
通讯作者：
Iwasaki, Akiko
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.