Early exercise improves cerebral blood flow through increased angiogenesis in experimental stroke rat model.
Early exercise improves cerebral blood flow through increased angiogenesis in experimental stroke rat model.
复制标题
早期运动通过增加实验性中风大鼠模型的血管生成来改善脑血流量
DOI：
10.1186/1743-0003-10-43
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发表时间：
2013-04-26
影响因子：
5.1
通讯作者：
Hu Y
中科院分区：
文献类型：
--
作者：
Zhang P;Yu H;Zhou N;Zhang J;Wu Y;Zhang Y;Bai Y;Jia J;Zhang Q;Tian S;Wu J;Hu Y
卒中后早期运动促进血管生成并增加微血管密度。然而，这些新形成的血管是否确实能形成功能性血管并改善受损脑区的脑血流（CBF）仍不清楚。本研究旨在确定早期运动对缺血区血管生成和脑血流的影响。
成年雄性Sprague Dawley大鼠大脑中动脉闭塞（MCAO）90分钟，并在24小时后随机分为早期运动组和非运动对照组。两周后，通过激光散斑血流仪（LSF）测定缺血区的脑血流。同时，分别采用免疫组织化学、2,3,5 - 氯化三苯基四氮唑（TTC）染色和蛋白质印迹法检测微血管密度、tie - 2、总Akt和磷酸化Akt（p - Akt）的表达以及梗死体积。通过七分法评估功能。
我们的结果显示，与非运动组相比，早期运动组缺血区的脑血流、血管密度以及Tie - 2、p - Akt的表达更高。与这些结果一致的是，早期运动组大鼠的梗死体积明显减小，功能预后优于非运动组。
我们的结果表明，MCAO后早期运动可改善缺血区的脑血流，减少梗死体积并促进功能恢复，其潜在机制与缺血皮质的血管生成相关。
BackgroundEarly exercise after stroke promoted angiogenesis and increased microvessles density. However, whether these newly formatted vessels indeed give rise to functional vascular and improve the cerebral blood flow (CBF) in impaired brain region is still unclear. The present study aimed to determine the effect of early exercise on angiogenesis and CBF in ischemic region.MethodsAdult male Sprague Dawley rats were subjected to 90 min middle cerebral artery occlusion(MCAO)and randomly divided into early exercise and non-exercised control group 24 h later. Two weeks later, CBF in ischemic region was determined by laser speckle flowmetry(LSF). Meantime, micro vessels density, the expression of tie-2, total Akt and phosphorylated Akt (p-Akt), and infarct volume were detected with immunohistochemistry, 2,3,5 triphenyltetrazolium chloride (TTC) staining and western blotting respectively. The function was evaluated by seven point’s method.ResultsOur results showed that CBF, vessel density and expression of Tie-2, p-Akt in ischemic region were higher in early exercise group compared with those in non-exercise group. Consistent with these results, rats in early exercise group had a significantly reduced infarct volume and better functional outcomes than those in non-exercise group.ConclusionsOur results indicated that early exercise after MCAO improved the CBF in ischemic region, reduced infarct volume and promoted the functional outcomes, the underlying mechanism was correlated with angiogenesis in the ischemic cortex.
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影响因子：
6.9
作者：
Gao X;Zhang H;Steinberg G;Zhao H
通讯作者：
Zhao H
影响因子：
37.8
作者：
Cheng XW;Kuzuya M;Kim W;Song H;Hu L;Inoue A;Nakamura K;Di Q;Sasaki T;Tsuzuki M;Shi GP;Okumura K;Murohara T
通讯作者：
Murohara T
影响因子：
4.2
作者：
Guo, Xinbin;Liu, Li;Zhang, Jianning
通讯作者：
Zhang, Jianning
影响因子：
6.3
作者：
Gavin, T. P.;Drew, J. L.;Hickner, R. C.
通讯作者：
Hickner, R. C.
发育和病理过程中的脑血管生成：中风后神经血管损伤和血管生成恢复。
DOI：
10.1111/j.1742-4658.2009.07176.x
发表时间：
2009-09
期刊：
The FEBS journal
影响因子：
--
作者：
Arai K;Jin G;Navaratna D;Lo EH
通讯作者：
Lo EH