Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia.
Releasing incompatible males drives strong suppression across populations of wild and Wolbachia-carrying Aedes aegypti in Australia.
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释放不兼容的男性在澳大利亚的野生和沃尔巴契亚岛伊德斯埃及埃及埃及的人群中进行了强烈的抑制。
DOI：
10.1073/pnas.2106828118
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发表时间：
2021-10-12
影响因子：
11.1
通讯作者：
Ritchie SA
中科院分区：
文献类型：
--
作者：
Beebe NW;Pagendam D;Trewin BJ;Boomer A;Bradford M;Ford A;Liddington C;Bondarenco A;De Barro PJ;Gilchrist J;Paton C;Staunton KM;Johnson B;Maynard AJ;Devine GJ;Hugo LE;Rasic G;Cook H;Massaro P;Snoad N;Crawford JE;White BJ;Xi Z;Ritchie SA
由于超过40%的人类面临登革热、黄热病、基孔肯雅热和寨卡等蚊媒疾病的风险，开发环保的蚊虫控制工具至关重要。携带沃尔巴克氏体细菌的繁殖不相容的雄性蚊子的释放可以驱动杀死卵的交配事件。通过在澳大利亚北方重复的处理和控制实验，定期释放感染白纹伊蚊Wolbachia的埃及伊蚊雄性，显示出在野生型（无Wolbachia）和携带wMel-Wolbachia的埃及伊蚊嵌合体种群中驱动强烈的种群抑制。埃及人。在示范不同的沃尔巴克氏体菌株在该领域之间的双向不相容性，我们还表明，一个赛季的抑制实验也可以显示到下一个赛季的持续影响。释放不育或不相容的雄性昆虫是农业系统中经过验证的种群管理方法，具有彻底改变蚊子控制的潜力。通过与Verily生命科学团队的合作，我们在澳大利亚北方的一项重复治疗对照田间试验中评估了与蚊子载体埃及伊蚊不相容的昆虫技术（IIT）。回交美国株Ae。携带来自白纹伊蚊的沃尔巴克氏体wAlbB的埃及伊蚊与当地菌株，我们产生了与野生型（无沃尔巴克氏体）和wMel-沃尔巴克氏体Ae不相容的wAlbB 2-F4菌株。现在存在于北昆士兰州的埃及人。使用Verily性别分选技术将wAlbB 2-F4菌株与雌性分离的雄性进行人工大规模饲养，以在田间获得不可检测的雌性污染。在社区的同意下，我们将总共300万IIT男性送到三个孤立的景观中，每个景观有200多所房子，每周三次在20周内释放500名男性。检测初始过洪比5：1和10：1之间，强大的人口下降远远超过80%，检测到在所有治疗景观相比，控制。通过下一个季节的监测，观察持续的影响，看到一个处理景观没有成年Ae。埃及在赛季初。第二个景观显示成年人减少，第三个完全恢复。这些令人鼓舞的结果抑制了野生型和wMel-Ae。埃及伊蚊证实了双向不亲和性在田间环境中的实用性，表明IIT是稳健的，并表明从人类居住的景观中去除这种虫媒病毒载体是可以实现的。
With over 40% of humans at risk from mosquito-borne diseases such as dengue, yellow fever, chikungunya, and Zika, the development of environmentally friendly mosquito-control tools is critical. The release of reproductively incompatible male mosquitoes carrying a Wolbachia bacterium can drive mating events that kill the eggs. Through replicated treatment and control experiments in northern Australia, regular releases of Aedes aegypti males infected with a Wolbachia from Aedes albopictus was shown to drive strong population suppression in mosaic populations of wild-type (no Wolbachia) and wMel-Wolbachia–carrying Ae. aegypti. In a demonstration of bidirectional incompatibility between different Wolbachia strains in the field, we also demonstrate that one season’s suppression experiment can also show an ongoing effect into the following season. Releasing sterile or incompatible male insects is a proven method of population management in agricultural systems with the potential to revolutionize mosquito control. Through a collaborative venture with the “Debug” Verily Life Sciences team, we assessed the incompatible insect technique (IIT) with the mosquito vector Aedes aegypti in northern Australia in a replicated treatment control field trial. Backcrossing a US strain of Ae. aegypti carrying Wolbachia wAlbB from Aedes albopictus with a local strain, we generated a wAlbB2-F4 strain incompatible with both the wild-type (no Wolbachia) and wMel-Wolbachia Ae. aegypti now extant in North Queensland. The wAlbB2-F4 strain was manually mass reared with males separated from females using Verily sex-sorting technologies to obtain no detectable female contamination in the field. With community consent, we delivered a total of three million IIT males into three isolated landscapes of over 200 houses each, releasing ∼50 males per house three times a week over 20 wk. Detecting initial overflooding ratios of between 5:1 and 10:1, strong population declines well beyond 80% were detected across all treatment landscapes when compared to controls. Monitoring through the following season to observe the ongoing effect saw one treatment landscape devoid of adult Ae. aegypti early in the season. A second landscape showed reduced adults, and the third recovered fully. These encouraging results in suppressing both wild-type and wMel-Ae. aegypti confirms the utility of bidirectional incompatibility in the field setting, show the IIT to be robust, and indicate that the removal of this arbovirus vector from human-occupied landscapes may be achievable.
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影响因子：
3.8
作者：
Johnson BJ;Mitchell SN;Paton CJ;Stevenson J;Staunton KM;Snoad N;Beebe N;White BJ;Ritchie SA
通讯作者：
Ritchie SA
影响因子：
3.1
作者：
FRANZ, G;GENCHEVA, E;KERREMANS, P
通讯作者：
KERREMANS, P
影响因子：
16.6
作者：
Galizi R;Doyle LA;Menichelli M;Bernardini F;Deredec A;Burt A;Stoddard BL;Windbichler N;Crisanti A
通讯作者：
Crisanti A
影响因子：
4.1
作者：
Hoffmann AA;Ross PA;Rašić G
通讯作者：
Rašić G
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y