Identification of soybean genes related to fatty acid content based on a soybean genome collinearity analysis
Identification of soybean genes related to fatty acid content based on a soybean genome collinearity analysis
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基于大豆基因组共线性分析鉴定与脂肪酸含量相关的大豆基因
DOI：
10.1111/pbr.12733
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发表时间：
2019-07
期刊：
影响因子：
2
通讯作者：
Qi Zhaoming
中科院分区：
文献类型：
--
作者：
Wang Xinyu;Li Qingying;Zhang Qi;Huang Shiyu;Yu Jingyao;Qin Hongtao;Qi Huidong;Li Yilin;Li Yingying;Yin Zhengong;Han Xue;Wu Xiaoxia;Xin Dawei;Chen Qingshan;Qi Zhaoming
大豆种子脂肪酸含量是食品、饲料和工业用大豆生产的重要考虑因素。本研究利用MCScanX对大豆全基因组进行分析，生成共线性块，然后利用该块评估SoyBase数据库中大豆脂肪酸数量性状位点（QTL）之间的共线性。鉴定出位于Gm06、Gm07和Gm10片段的hub‐qtl。利用京都基因与基因组百科全书和基因本体数据库对hub‐QTL区域的基因进行分析，鉴定出17个与大豆脂肪酸含量相关的候选基因。从染色体片段替换细胞系文库中选择两种脂肪酸含量不同的细胞系和一个重复亲本，进行实时定量聚合酶链反应（qRT - PCR）检测，以验证候选基因的表达模式。4个基因与大豆总脂肪酸含量有关，3个基因与特定脂肪酸类型含量有关。本研究结果可能为大豆脂肪酸qtl /基因的精细定位提供参考。
Seed fatty acid content is an important consideration for soybean produced for food, feed, and industrial applications. In this study, MCScanX was used to analyze the entire soybean genome to generate a collinearity block, which was then used to assess the collinearity among the soybean fatty acid quantitative trait loci (QTL) in the SoyBase database. The hub‐QTLs located in the Gm06, Gm07, and Gm10 segments were identified. The Kyoto Encyclopedia of Genes and Genomes and gene ontology databases were used to analyze the genes in hub‐QTL regions, resulting in the identification of 17 candidate genes related to soybean fatty acid content. Two lines with different fatty acid contents and a recurrent parent were selected from a chromosome segment substitution line library for a subsequent quantitative real‐time polymerase chain reaction (qRT‐PCR) assay to verify the candidate gene expression patterns. Four genes were related to the total soybean fatty acid content, while three genes were related to the content of specific fatty acid types. The results of this study may be relevant for the fine mapping of soybean fatty acid QTLs/genes.
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