Soybean GmbZIP123 gene enhances lipid content in the seeds of transgenic Arabidopsis plants.
Soybean GmbZIP123 gene enhances lipid content in the seeds of transgenic Arabidopsis plants.
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DOI：
10.1093/jxb/ert238
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发表时间：
2013-11
影响因子：
6.9
通讯作者：
Zhang JS
中科院分区：
文献类型：
--
作者：
Song QX;Li QT;Liu YF;Zhang FX;Ma B;Zhang WK;Man WQ;Du WG;Wang GD;Chen SY;Zhang JS
大豆是最重要的油料作物之一，大豆种子脂肪含量的显著增加将促进世界植物油生产。虽然高等植物中脂类生物合成的途径已经被揭示，但我们对控制脂类积累的调控机制的了解仍然有限。在这项研究中，我们确定了87个转录因子基因在大豆种子中的脂质积累阶段具有较高的丰度。选择其中一个基因--ZIP 123，以进一步研究其在调节脂质积累中的功能。过表达的ZIP 123增强了转基因拟南芥植物种子中的脂质含量。该转基因通过直接与两个蔗糖转运蛋白基因（SUC 1和SUC 5）和三个细胞壁转化酶基因（cwINV 1、cwINV 3和cwINV 6）的启动子结合来促进这些基因的表达。因此，转ZIP 123基因植株的角果中细胞壁转化酶活性和糖转运都得到了提高。在转ZIP 123基因植物的种子中也发现了更高水平的葡萄糖、果糖和蔗糖。这些结果表明，ZIP 123可能通过控制糖从光合自养组织转运到种子中来参与调节大豆种子中的脂质积累。这项研究为种子中脂质积累的调控机制提供了新的见解，并可能通过对ZIP 123基因的遗传操作来促进大豆和其他油料作物的产油。
Soybean is one of most important oil crops and a significant increase in lipid content in soybean seeds would facilitate vegetable oil production in the world. Although the pathways for lipid biosynthesis in higher plants have been uncovered, our understanding of regulatory mechanism controlling lipid accumulation is still limited. In this study, we identified 87 transcription factor genes with a higher abundance at the stage of lipid accumulation in soybean seeds. One of these genes, GmbZIP123, was selected to further study its function in regulation of lipid accumulation. Overexpression of GmbZIP123 enhanced lipid content in the seeds of transgenic Arabidopsis thaliana plants. The GmbZIP123 transgene promoted expression of two sucrose transporter genes (SUC1 and SUC5) and three cell-wall invertase genes (cwINV1, cwINV3, and cwINV6) by binding directly to the promoters of these genes. Consistently, the cell-wall invertase activity and sugar translocation were all enhanced in siliques of GmbZIP123 transgenic plants. Higher levels of glucose, fructose, and sucrose were also found in seeds of GmbZIP123 transgenic plants. These results suggest that GmbZIP123 may participate in regulation of lipid accumulation in soybean seeds by controlling sugar transport into seeds from photoautotrophic tissues. This study provides novel insights into the regulatory mechanism for lipid accumulation in seeds and may facilitate improvements in oil production in soybean and other oil crops through genetic manipulation of the GmbZIP123 gene.
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影响因子：
7.4
作者：
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通讯作者：
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