Regulation of UVR8 photoreceptor dimer/monomer photo-equilibrium in Arabidopsis plants grown under photoperiodic conditions.
Regulation of UVR8 photoreceptor dimer/monomer photo-equilibrium in Arabidopsis plants grown under photoperiodic conditions.
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DOI：
10.1111/pce.12724
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发表时间：
2016-08
期刊：
影响因子：
--
通讯作者：
Jenkins GI
中科院分区：
文献类型：
--
作者：
Findlay KM;Jenkins GI
UV抗性基因座8（UVR 8）感光体特异性介导对UV-B的光形态发生反应。光感受诱导二聚体UVR 8解离成单体以引发反应。然而，在光周期条件下生长的植物中的二聚体/单体状态的调节尚未被研究。在这里，我们表明，UVR 8在受控环境和自然光照下，在昼夜光周期中生长的植物中建立了二聚体/单体光平衡。光平衡由二聚体的光感受和暗回复的相对速率决定。对UV-B光形态发生抑制剂1（RUP 1）和RUP 2突变体的实验表明，这些蛋白质在调节光平衡方面至关重要，因为它们促进二聚体的回复。在日光下生长的植物中，UVR 8光平衡与UV B的低环境注量率（高达1.5 μmol m−2 s−1）密切相关，而不是较高的注量率或光合有效辐射量。此外，在较低温度下，单体转化为二聚体的速率降低，从而在约8-10 °C下促进单体相对水平的增加。因此，在光周期生长条件下，UVR 8的行为不像一个简单的UV-B开关，而是建立一个由UV-B调节并受温度影响的二聚体/单体光平衡。光感受器UVR 8吸收UV-B诱导二聚体解离，形成引发光形态发生UV-B响应的单体。然而，在光周期条件下生长的植物中的二聚体/单体状态的调节尚未被研究。在这里，我们表明，在受控环境和自然日光的光周期条件下，UVR 8的行为不像一个简单的UV B开关，而是建立了一个由UV B调节并受温度影响的二聚体/单体光平衡。
The UV RESISTANCE LOCUS 8 (UVR8) photoreceptor specifically mediates photomorphogenic responses to UV‐B. Photoreception induces dissociation of dimeric UVR8 into monomers to initiate responses. However, the regulation of dimer/monomer status in plants growing under photoperiodic conditions has not been examined. Here we show that UVR8 establishes a dimer/monomer photo‐equilibrium in plants growing in diurnal photoperiods in both controlled environments and natural daylight. The photo‐equilibrium is determined by the relative rates of photoreception and dark‐reversion to the dimer. Experiments with mutants in REPRESSOR OF UV‐B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2 show that these proteins are crucial in regulating the photo‐equilibrium because they promote reversion to the dimer. In plants growing in daylight, the UVR8 photo‐equilibrium is most strongly correlated with low ambient fluence rates of UV‐B (up to 1.5 μmol m−2 s−1), rather than higher fluence rates or the amount of photosynthetically active radiation. In addition, the rate of reversion of monomer to dimer is reduced at lower temperatures, promoting an increase in the relative level of monomer at approximately 8–10 °C. Thus, UVR8 does not behave like a simple UV‐B switch under photoperiodic growth conditions but establishes a dimer/monomer photo‐equilibrium that is regulated by UV‐B and also influenced by temperature. UV‐B absorption by the photoreceptor UVR8 induces dimer dissociation, forming monomers that initiate photomorphogenic UV‐B responses. However, the regulation of dimer/monomer status in plants growing under photoperiodic conditions has not been examined. Here we show that UVR8 does not behave like a simple UV‐B switch under photoperiodic conditions in both controlled environments and natural daylight, but establishes a dimer/monomer photo‐equilibrium that is regulated by UV‐B and influenced by temperature.
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作者：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
--
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