Chromatin architectures at fission yeast transcriptional promoters and replication origins.
Chromatin architectures at fission yeast transcriptional promoters and replication origins.
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DOI：
10.1093/nar/gks351
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发表时间：
2012-08
影响因子：
14.9
通讯作者：
Buck MJ
中科院分区：
文献类型：
--
作者：
Givens RM;Lai WK;Rizzo JM;Bard JE;Mieczkowski PA;Leatherwood J;Huberman JA;Buck MJ
我们使用微球菌核酸酶(MNase)消化，然后进行深度测序，以获得比以前在分裂酵母中获得的核小体位置更高的分辨率图谱。我们的数据证实了在分裂酵母中存在一个异常短的平均核小体重复长度∼152 bp，并且转录起始点(TSS)与核小体枯竭区域(NDR)、下游有序的核小体阵列和上游排列不规则的核小体相关。此外，我们发现在TSS附近根据染色质配置聚在一起的八组基因中，有四组相关功能丰富。在复制起点，我们的数据揭示了复制前复合体(pre-RC)蛋白在大的NDR中的不对称定位--这一特征在分裂和发芽酵母中是保守的，因此很可能在其他真核生物中也是保守的。
We have used micrococcal nuclease (MNase) digestion followed by deep sequencing in order to obtain a higher resolution map than previously available of nucleosome positions in the fission yeast, Schizosaccharomyces pombe. Our data confirm an unusually short average nucleosome repeat length, ∼152 bp, in fission yeast and that transcriptional start sites (TSSs) are associated with nucleosome-depleted regions (NDRs), ordered nucleosome arrays downstream and less regularly spaced upstream nucleosomes. In addition, we found enrichments for associated function in four of eight groups of genes clustered according to chromatin configurations near TSSs. At replication origins, our data revealed asymmetric localization of pre-replication complex (pre-RC) proteins within large NDRs—a feature that is conserved in fission and budding yeast and is therefore likely to be conserved in other eukaryotic organisms.
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影响因子：
12.3
作者：
Kaplan N;Hughes TR;Lieb JD;Widom J;Segal E
通讯作者：
Segal E
影响因子：
4.5
作者：
Buchanan L;Durand-Dubief M;Roguev A;Sakalar C;Wilhelm B;Strålfors A;Shevchenko A;Aasland R;Shevchenko A;Ekwall K;Francis Stewart A
通讯作者：
Francis Stewart A
影响因子：
21.3
作者：
Feng, Wenyi;Collingwood, David;Boeck, Max E;Fox, Lindsay A;Alvino, Gina M;Fangman, Walton L;Raghuraman, Mosur K;Brewer, Bonita J
通讯作者：
Brewer, Bonita J
影响因子：
10.5
作者：
Eaton, Matthew L.;Galani, Kyriaki;MacAlpine, David M.
通讯作者：
MacAlpine, David M.
影响因子：
11.4
作者：
Durand-Dubief, Mickael;Persson, Jenna;Ekwall, Karl
通讯作者：
Ekwall, Karl