Cdc42 and Par proteins stabilize dynamic adherens junctions in the Drosophila neuroectoderm through regulation of apical endocytosis.
Cdc42 and Par proteins stabilize dynamic adherens junctions in the Drosophila neuroectoderm through regulation of apical endocytosis.
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DOI：
10.1083/jcb.200807020
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发表时间：
2008-12-15
期刊：
影响因子：
--
通讯作者：
Tepass U
中科院分区：
文献类型：
--
作者：
Harris KP;Tepass U
细胞重排需要细胞-细胞接触的动态变化来维持组织的完整性。我们研究了Cdc 42在维持果蝇神经外胚层上皮细胞粘附连接（AJs）和顶端极性中的功能。大约三分之一的细胞通过侵入离开上皮并成为神经母细胞。Cdc 42受损的胚胎在成神经细胞侵入过程中失去了神经外胚层中的AJs。相反，当成神经细胞的形成受到抑制，AJs保持尽管Cdc 42功能的损失。Cdc 42功能的丧失导致顶端蛋白的内吞摄取增加，包括AJ稳定性所需的顶端极性因子如碎屑。此外，Cdc 42在调节内吞运输中具有第二功能，因为它是顶端货物从早期到晚期内体的进展所必需的。Par复合物在控制顶端蛋白的内吞作用中充当Cdc 42的效应子。这项研究揭示了顶端极性蛋白和内吞作用之间的功能相互作用，这对稳定动态基底外侧AJs至关重要。
Cell rearrangements require dynamic changes in cell–cell contacts to maintain tissue integrity. We investigated the function of Cdc42 in maintaining adherens junctions (AJs) and apical polarity in the Drosophila melanogaster neuroectodermal epithelium. About one third of cells exit the epithelium through ingression and become neuroblasts. Cdc42-compromised embryos lost AJs in the neuroectoderm during neuroblast ingression. In contrast, when neuroblast formation was suppressed, AJs were maintained despite the loss of Cdc42 function. Loss of Cdc42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs, which are required for AJ stability. In addition, Cdc42 has a second function in regulating endocytotic trafficking, as it is required for the progression of apical cargo from the early to the late endosome. The Par complex acts as an effector for Cdc42 in controlling the endocytosis of apical proteins. This study reveals functional interactions between apical polarity proteins and endocytosis that are critical for stabilizing dynamic basolateral AJs.
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影响因子：
19
作者：
D'Souza-Schorey, C
通讯作者：
D'Souza-Schorey, C
影响因子：
4.5
作者：
Cohen, D;Müsch, A;Rodriguez-Boulan, E
通讯作者：
Rodriguez-Boulan, E
影响因子：
64.5
作者：
Garrett, WS;Chen, LM;Mellman, I
通讯作者：
Mellman, I
影响因子：
64.5
作者：
Entchev, EV;Schwabedissen, A;González-Gaitán, M
通讯作者：
González-Gaitán, M
DOI：
10.1002/neu.480241005
发表时间：
1993-10-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
CAMPOSORTEGA, JA
通讯作者：
CAMPOSORTEGA, JA