Essential role of peripheral node addressin in lymphocyte homing to nasal-associated lymphoid tissues and allergic immune responses.
Essential role of peripheral node addressin in lymphocyte homing to nasal-associated lymphoid tissues and allergic immune responses.
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DOI：
10.1084/jem.20101786
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发表时间：
2011-05-09
期刊：
影响因子：
--
通讯作者：
Kawashima H
中科院分区：
文献类型：
--
作者：
Ohmichi Y;Hirakawa J;Imai Y;Fukuda M;Kawashima H
传统T细胞对NALT的归巢主要依赖于l -选择素- pnad的相互作用，而调节性T细胞的归巢也依赖于PSGL1-和cd44介导的粘附。鼻相关淋巴组织（NALT）是一种粘膜免疫组织，对吸入抗原提供免疫应答。在NALT中，淋巴细胞与高内皮小静脉（HEVs）之间的特异性相互作用介导了淋巴细胞归巢。与其他粘膜淋巴组织中的hev相比，NALT hev强烈表达外周节点地址蛋白（PNAds），该蛋白携带可被单克隆抗体MECA-79识别的硫酸化聚糖。我们利用n -乙酰氨基葡萄糖-6- o -磺基转移酶（GlcNAc6ST） 1和GlcNAc6ST-2双敲除（DKO）小鼠研究了PNAd在淋巴细胞归巢NALT中的作用。DKO小鼠NALT hev中PNAd的表达被消除。短期归巢实验表明，DKO小鼠的淋巴细胞归巢NALT减少了90%。抗原特异性IgE的产生和鼻用卵清蛋白应答的打喷嚏次数也大大减少。与此一致的是，DKO小鼠的NALT显示IL-4的产生减少，IL-10的产生增加，CD4+CD25+调节性T细胞（Treg细胞）的增加。与CD4+CD25 -传统T细胞相比，CD4+CD25+ Treg细胞对NALT的归巢较少依赖于l -选择素- pnad相互作用，但部分依赖于PSGL-1 （p -选择素糖蛋白配体1）和CD44。这些结果表明PNAd对淋巴细胞归巢NALT和鼻腔过敏反应至关重要。
Homing of conventional T cells to NALT is primarily dependent on L-selectin–PNAd interactions, whereas homing of regulatory T cells also depends on PSGL1- and CD44-mediated adhesion. Nasal-associated lymphoid tissue (NALT) is a mucosal immune tissue that provides immune responses against inhaled antigens. Lymphocyte homing to NALT is mediated by specific interactions between lymphocytes and high endothelial venules (HEVs) in NALT. In contrast to HEVs in other mucosal lymphoid tissues, NALT HEVs strongly express peripheral node addressins (PNAds) that bear sulfated glycans recognized by the monoclonal antibody MECA-79. We investigated the role of PNAd in lymphocyte homing to NALT using sulfotransferase N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST) 1 and GlcNAc6ST-2 double knockout (DKO) mice. The expression of PNAd in NALT HEVs was eliminated in DKO mice. Short-term homing assays indicated that lymphocyte homing to NALT was diminished by 90% in DKO mice. Production of antigen-specific IgE and the number of sneezes in response to nasally administered ovalbumin were also substantially diminished. Consistently, the NALT of DKO mice showed reduced production of IL-4 and increased production of IL-10 together with an increase in CD4+CD25+ regulatory T cells (Treg cells). Compared with the homing of CD4+CD25− conventional T cells, the homing of CD4+CD25+ Treg cells to NALT was less dependent on the L-selectin–PNAd interaction but was partially dependent on PSGL-1 (P-selectin glycoprotein ligand 1) and CD44. These results demonstrate that PNAd is essential for lymphocyte homing to NALT and nasal allergic responses.
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影响因子：
64.8
作者：
GALLATIN, WM;WEISSMAN, IL;BUTCHER, EC
通讯作者：
BUTCHER, EC
影响因子：
56.9
作者：
Butcher, EC;Picker, LJ
通讯作者：
Picker, LJ
DOI：
10.1084/jem.183.3.1119
发表时间：
1996-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
DeGrendele HC;Estess P;Picker LJ;Siegelman MH
通讯作者：
Siegelman MH
DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
4.8
作者：
Hiraoka, N;Kawashima, H;Fukuda, M
通讯作者：
Fukuda, M