lncRsp1, a long noncoding RNA, influences Fgsp1 expression and sexual reproduction in Fusarium graminearum.
lncRsp1, a long noncoding RNA, influences Fgsp1 expression and sexual reproduction in Fusarium graminearum.
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lncRsp1是一种长非编码RNA，影响禾谷镰刀菌中Fgsp1的表达和有性生殖
DOI：
10.1111/mpp.13160
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发表时间：
2022-03
影响因子：
4.9
通讯作者：
Lin Y
中科院分区：
文献类型：
--
作者：
Wang J;Zeng W;Cheng J;Xie J;Fu Y;Jiang D;Lin Y
摘要长非编码RNA(LncRNAs)在许多生物过程中是基因表达的重要调节因子，但其生物学功能仍不清楚，尤其是在真菌中。禾谷镰刀菌是引起小麦和大麦赤霉病的重要病原菌。在我们先前的RNA测序(RNA-Seq)研究中，我们发现lncRsp1是一个位于糖转运蛋白基因Fgsp1上游+99bp的LncRNA，具有相同的转录方向。功能研究表明，ΔlncRsp1和ΔFgsp1在生长和分生方面正常，但在子囊孢子排出和对小麦胚芽鞘的毒力方面存在缺陷。此外，lncRsp1和Fgsp1对几个脱氧雪腐镰刀菌烯醇(DON)生物合成基因TRI4、TRI5、TRI6和TRI13的表达以及DON的产生都有负调控作用。进一步的分析表明，在有性生殖阶段，lncRsp1的过表达增强了子囊孢子的释放能力，提高了Fgsp1基因的表达水平，而lncRsp1沉默的菌株则减少了子囊孢子的释放，抑制了Fgsp1的表达。此外，lncRsp1互补菌株lncRsp1-LC-1和lncRsp1-LC-2将子囊孢子释放恢复到野生型菌株PH-1的水平。综上所述，我们的结果揭示了lncRsp1和Fgsp1在禾谷镰刀菌中的不同和特异的功能，主要证明了lncRsp1通过调节Fgsp1的表达来影响子囊孢子的释放。
Abstract Long noncoding RNAs (lncRNAs) are crucial regulators of gene expression in many biological processes, but their biological functions remain largely unknown, especially in fungi. Fusarium graminearum is an important pathogen that causes the destructive disease Fusarium head blight (FHB) or head scab disease on wheat and barley. In our previous RNA sequencing (RNA‐Seq) study, we discovered that lncRsp1 is an lncRNA that is located +99 bp upstream of a putative sugar transporter gene, Fgsp1, with the same transcription direction. Functional studies revealed that ΔlncRsp1 and ΔFgsp1 were normal in growth and conidiation but had defects in ascospore discharge and virulence on wheat coleoptiles. Moreover, lncRsp1 and Fgsp1 were shown to negatively regulate the expression of several deoxynivalenol (DON) biosynthesis genes, TRI4, TRI5, TRI6, and TRI13, as well as DON production. Further analysis showed that the overexpression of lncRsp1 enhanced the ability of ascospore release and increased the mRNA expression level of the Fgsp1 gene, while lncRsp1‐silenced strains reduced ascospore discharge and inhibited Fgsp1 expression during the sexual reproduction stage. In addition, the lncRsp1 complementary strains lncRsp1‐LC‐1 and lncRsp1‐LC‐2 restored ascospore discharge to the level of the wild‐type strain PH‐1. Taken together, our results reveal the distinct and specific functions of lncRsp1 and Fgsp1 in F. graminearum and principally demonstrate that lncRsp1 can affect the release of ascospores by regulating the expression of Fgsp1.
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影响因子：
3.6
作者：
Donaldson ME;Saville BJ
通讯作者：
Saville BJ
影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
3.5
作者：
Bluhm, B. H.;Zhao, X.;Dunkle, L. D.
通讯作者：
Dunkle, L. D.
影响因子：
4.1
作者：
da Rocha Campos, Andre Narvaes;Costa, Mauricio Dutra
通讯作者：
Costa, Mauricio Dutra
影响因子：
4.2
作者：
Audenaert K;Vanheule A;Höfte M;Haesaert G
通讯作者：
Haesaert G