Genome-enabled discovery of candidate virulence loci in Striga hermonthica, a devastating parasite of African cereal crops.
Genome-enabled discovery of candidate virulence loci in Striga hermonthica, a devastating parasite of African cereal crops.
复制标题
DOI：
10.1111/nph.18305
复制
发表时间：
2022-10
期刊：
影响因子：
9.4
通讯作者：
Scholes, Julie D.
中科院分区：
文献类型：
--
作者：
Qiu, Suo;Bradley, James M.;Zhang, Peijun;Chaudhuri, Roy;Blaxter, Mark;Butlin, Roger K.;Scholes, Julie D.
关键词：
寄生虫已经进化出蛋白质，毒力因子（VF），促进植物定殖，但VF介导的寄生植物-宿主相互作用知之甚少。Striga hermonthica是撒哈拉以南非洲谷物宿主的专性根寄生植物，造成毁灭性的产量损失。了解疟原虫VF的分子特性和等位变异对于培育抗性和延缓寄生虫毒力进化至关重要。我们组装了S. hermonthica基因组，并使用计算机预测鉴定了分泌蛋白。对易感和强抗性水稻宿主上生长的寄生虫进行合并测序，使我们能够扫描抗性宿主选择的位点，这些位点提高了成功定植的等位基因频率。38个蛹分泌的VF具有非常不同的等位基因频率，其功能包括宿主细胞壁修饰、蛋白酶或蛋白酶抑制剂和激酶活性。这些候选基因座具有比基因组背景显著更高的Tajima's D，与平衡选择一致。我们的研究结果揭示了不同的策略使用S. hermonthica克服不同层次的主机电阻。了解通过平衡选择维持毒力基因座的变异对于管理毒力进化作为可持续控制策略的一部分至关重要。另见韦斯特伍德对这篇文章的评论，236：316-318。
Parasites have evolved proteins, virulence factors (VFs), that facilitate plant colonisation, however VFs mediating parasitic plant–host interactions are poorly understood. Striga hermonthica is an obligate, root‐parasitic plant of cereal hosts in sub‐Saharan Africa, causing devastating yield losses. Understanding the molecular nature and allelic variation of VFs in S. hermonthica is essential for breeding resistance and delaying the evolution of parasite virulence. We assembled the S. hermonthica genome and identified secreted proteins using in silico prediction. Pooled sequencing of parasites growing on a susceptible and a strongly resistant rice host allowed us to scan for loci where selection imposed by the resistant host had elevated the frequency of alleles contributing to successful colonisation. Thirty‐eight putatively secreted VFs had very different allele frequencies with functions including host cell wall modification, protease or protease inhibitor and kinase activities. These candidate loci had significantly higher Tajima's D than the genomic background, consistent with balancing selection. Our results reveal diverse strategies used by S. hermonthica to overcome different layers of host resistance. Understanding the maintenance of variation at virulence loci by balancing selection will be critical to managing the evolution of virulence as part of a sustainable control strategy. See also the Commentary on this article by Westwood, 236: 316–318.
登录
查看更多内容
影响因子：
5.3
作者：
Bleischwitz M;Albert M;Fuchsbauer HL;Kaldenhoff R
通讯作者：
Kaldenhoff R
影响因子：
11.6
作者：
Ishida, Juliane K.;Wakatake, Takanori;Shirasu, Ken
通讯作者：
Shirasu, Ken
影响因子：
12.3
作者：
Haas, Brian J.;Salzberg, Steven L.;Zhu, Wei;Pertea, Mihaela;Allen, Jonathan E.;Orvis, Joshua;White, Owen;Buell, C. Robin;Wortman, Jennifer R.
通讯作者：
Wortman, Jennifer R.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
7
作者：
Kajitani R;Toshimoto K;Noguchi H;Toyoda A;Ogura Y;Okuno M;Yabana M;Harada M;Nagayasu E;Maruyama H;Kohara Y;Fujiyama A;Hayashi T;Itoh T
通讯作者：
Itoh T