Significance of Cuscutain, a cysteine protease from Cuscuta reflexa, in host-parasite interactions.
Significance of Cuscutain, a cysteine protease from Cuscuta reflexa, in host-parasite interactions.
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DOI：
10.1186/1471-2229-10-227
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发表时间：
2010-10-22
影响因子：
5.3
通讯作者：
Kaldenhoff R
中科院分区：
文献类型：
--
作者：
Bleischwitz M;Albert M;Fuchsbauer HL;Kaldenhoff R
植物感染寄生杂草，如反枝菟丝子诱导宿主和寄生虫的形态以及生化变化。这些修饰可能是由蛋白质或基因活性的变化引起的。使用比较宏阵列方法，确定了在宿主附着位点特异性上调的菟丝子属基因。其中一种侵染特异性菟丝子基因编码半胱氨酸蛋白酶。异源表达，纯化和生化特性的蛋白质及其内在的抑制肽。吸器特异性酶被命名为cuscutain，与来自其他植物（例如木瓜）的类似蛋白质一致。菟丝子素及其抑制剂在宿主寄生虫相互作用过程中的作用进行了研究，通过外部应用的抑制剂悬浮液，诱导成功的感染事件显着减少。该研究提供了有关寄生植物与宿主相互作用期间分子事件的新信息。抑制菟丝子素半胱氨酸蛋白酶可为拮抗寄生植物提供手段。
Plant infestation with parasitic weeds like Cuscuta reflexa induces morphological as well as biochemical changes in the host and the parasite. These modifications could be caused by a change in protein or gene activity. Using a comparative macroarray approach Cuscuta genes specifically upregulated at the host attachment site were identified. One of the infestation specific Cuscuta genes encodes a cysteine protease. The protein and its intrinsic inhibitory peptide were heterologously expressed, purified and biochemically characterized. The haustoria specific enzyme was named cuscutain in accordance with similar proteins from other plants, e.g. papaya. The role of cuscutain and its inhibitor during the host parasite interaction was studied by external application of an inhibitor suspension, which induced a significant reduction of successful infection events. The study provides new information about molecular events during the parasitic plant - host interaction. Inhibition of cuscutain cysteine proteinase could provide means for antagonizing parasitic plants.
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影响因子：
3.8
作者：
Dvorák J;Mashiyama ST;Sajid M;Braschi S;Delcroix M;Schneider EL;McKerrow WH;Bahgat M;Hansell E;Babbitt PC;Craik CS;McKerrow JH;Caffrey CR
通讯作者：
Caffrey CR
影响因子：
2
作者：
Kaewpitoon, Natthawut;Laha, Thewarach;Sripa, Banchob
通讯作者：
Sripa, Banchob
DOI：
10.1073/pnas.90.7.3063
发表时间：
1993-04-01
影响因子：
11.1
作者：
KARRER, KM;PEIFFER, SL;DITOMAS, ME
通讯作者：
DITOMAS, ME
抑制消减杂交鉴定菟丝子感染期间薇甘菊差异表达基因
影响因子：
4.3
作者：
Li, Dong-Mei;Staehelin, Christian;Peng, Shao-Lin
通讯作者：
Peng, Shao-Lin
影响因子：
4.4
作者：
Castillejo MA;Maldonado AM;Dumas-Gaudot E;Fernández-Aparicio M;Susín R;Diego R;Jorrín JV
通讯作者：
Jorrín JV