An ethanol vapor chamber system for small animals.
An ethanol vapor chamber system for small animals.
复制标题
用于小动物的乙醇蒸汽室系统
DOI：
10.1016/j.jneumeth.2012.04.017
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发表时间：
2012-06-30
影响因子：
3
通讯作者：
Mason, Graeme F.
中科院分区：
文献类型：
--
作者：
Wang, Jie;Jiang, Lihong;Du, Hongying;Mason, Graeme F.
乙醇蒸汽室自1971年引入以来已被广泛用于酒精研究，并且这些系统的实现现在可商业化。在这里，我们提出了一个修改的腔室，可以建立在更低的成本和更简单的操作。用于大鼠的六室系统具有多个气泵。乙醇蒸汽水平通过空气流速、乙醇滴注速率和室内空气稀释进行调节，无需加热器或风扇。乙醇蒸汽浓度用呼吸测醉器测量，使用室内空气将蒸汽室输出稀释到呼吸测醉器的范围内。多个泵提供备用，以确保在主空气泵故障的情况下动物的存活。动物试验表明，血液乙醇的舒适和稳定的升高，乙醇蒸汽浓度的严格控制和从广泛的水平范围内选择的能力。乙醇蒸气的测量是快速和有效的。零件的成本是几千美元。该系统具有成本低、使用方便、测量乙醇蒸气浓度方便、成本低的特点。在我们的案例中，由于没有加热器和可能接触乙醇的电气部件，因此促进了机构的批准。
Ethanol vapor chambers have been utilized widely in alcohol research since their introduction in 1971, and implementations of these systems are now available commercially. Here, we present a modification of the chamber that can be built at lower cost and greater simplicity of operation. The six-chamber system for rats has multiple air pumps. Ethanol vapor levels are adjusted with the air flow rate, ethanol drip rate, and dilution with room air, without a heater or fans. Ethanol vapor concentrations are measured with a breathalyzer, using room air to dilute the vapor chamber output into the range of the breathalyzer. Multiple pumps provide backup to ensure animal survival in the case of failure of the primary air pump. Tests in animals demonstrated comfortable and stable elevation of blood ethanol, with tight control of the ethanol vapor concentrations and the ability to select from a broad range of levels. The ethanol vapor measurement was rapid and efficient. The parts cost was a few thousand U.S. dollars. This vapor chamber system features low cost, ease of use, and convenient and inexpensive measurement of ethanol vapor concentrations. The lack of a heater and electrical components that could come into contact with ethanol in our case facilitated institutional approval.
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影响因子：
10.6
作者：
Funk, Cindy K.;Zorrilla, Eric P.;Koob, George F.
通讯作者：
Koob, George F.
DOI：
10.1111/j.1530-0277.1986.tb05121.x
发表时间：
1986-08-01
期刊：
ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH
影响因子：
--
作者：
KARANIAN, J;YERGEY, J;SALEM, N
通讯作者：
SALEM, N
影响因子：
--
作者：
Gilpin, Nicholas W;Richardson, Heather N;Cole, Maury;Koob, George F
通讯作者：
Koob, George F
影响因子：
4.8
作者：
Rimondini, R;Arlinde, C;Heilig, M
通讯作者：
Heilig, M
影响因子：
3.8
作者：
FERKO, AP;BOBYOCK, E
通讯作者：
BOBYOCK, E