DNA barcoding reveals both known and novel taxa in the Albitarsis Group (Anopheles: Nyssorhynchus) of Neotropical malaria vectors.
DNA barcoding reveals both known and novel taxa in the Albitarsis Group (Anopheles: Nyssorhynchus) of Neotropical malaria vectors.
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DNA条形码揭示了新热带疟疾载体的白皮亚组（蚊子：Nyssorhynchus）中的已知和新型分类单元。
DOI：
10.1186/1756-3305-5-44
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发表时间：
2012-02-21
影响因子：
3.2
通讯作者：
Linton YM
中科院分区：
文献类型：
--
作者：
Ruiz-Lopez F;Wilkerson RC;Conn JE;McKeon SN;Levin DM;Quiñones ML;Póvoa MM;Linton YM
属于白纹伊蚊(按蚊目：Nyssorhynchus)的蚊子是新热带地区重要的疟疾媒介。该集团目前由6个已知物种组成，最近的研究表明，该集团内还有更多隐藏的生物多样性。DNA条形码已被认为是一种非常有用的物种识别工具，尽管它的区分性作用尚未在广泛地理分布的密切相关类群中得到验证。DNA条形码(线粒体细胞色素c氧化酶-COI的658bp)产生于565An。白化病S.L.过去20年在阿根廷、巴西、哥伦比亚、巴拉圭、特立尼达和委内瑞拉采集的标本，包括模式系列和模式地区的标本。在这里，我们测试了当前倡导的条形码方法的实用性，包括木村双参数距离模型(K2P)和邻居连接分析(NJ)，用于确定新热带白化蚊媒(按蚊：Nyssorhynchus)蚊子内的物种描述，并将结果与贝叶斯分析进行比较。通过条形码分析和贝叶斯系统发育分析来描述物种，完全同意。对565个序列(302个独特的单倍型)的分析解决了9个新泽西州树簇，节点内变异小于2%。种内平均变异(K2P)为0.009(范围0.002-0.014)，而种间平均差异为0.041(0.020-0.056)，高出数倍，支持已报道的“条形码差距”。这些结果完全支持阿尔比塔斯群中6个已知种的独立种地位。Albitarsis S.S.，An.Albitarsis F，An.教务长，一个。Janconae，An.Marajoara和An.Oryzalimnetes)，还支持先前检测到的两个谱系的物种水平状态。白云英属植物。白化病I(在此指定)。此外，我们强调了一个独特的线粒体谱系的存在接近一个。教务长和一名。Marajoara(An.Albitarsis H)来自巴西西南部的朗多尼亚和马托格罗索州。需要进一步的综合研究来确认这一谱系的状况。DNA条形码提供了一种可靠的方法来识别白化石群中密切相关的分类群中的已知和未发现的生物多样性。我们主张在未来的研究中使用它来阐明白化病组和新的线粒体谱系中所有8个物种的载体能力和各自的分布。在本研究中恢复。
Mosquitoes belonging to the Albitarsis Group (Anopheles: Nyssorhynchus) are of importance as malaria vectors across the Neotropics. The Group currently comprises six known species, and recent studies have indicated further hidden biodiversity within the Group. DNA barcoding has been proposed as a highly useful tool for species recognition, although its discriminatory utility has not been verified in closely related taxa across a wide geographic distribution. DNA barcodes (658 bp of the mtDNA Cytochrome c Oxidase - COI) were generated for 565 An. albitarsis s.l. collected in Argentina, Brazil, Colombia, Paraguay, Trinidad and Venezuela over the past twenty years, including specimens from type series and type localities. Here we test the utility of currently advocated barcoding methodologies, including the Kimura-two-parameter distance model (K2P) and Neighbor-joining analysis (NJ), for determining species delineation within mosquitoes of the Neotropical Albitarsis Group of malaria vectors (Anopheles: Nyssorhynchus), and compare results with Bayesian analysis. Species delineation through barcoding analysis and Bayesian phylogenetic analysis, fully concur. Analysis of 565 sequences (302 unique haplotypes) resolved nine NJ tree clusters, with less than 2% intra-node variation. Mean intra-specific variation (K2P) was 0.009 (range 0.002 - 0.014), whereas mean inter-specific divergence were several-fold higher at 0.041 (0.020 - 0.056), supporting the reported "barcoding gap". These results show full support for separate species status of the six known species in the Albitarsis Group (An. albitarsis s.s., An. albitarsis F, An. deaneorum, An. janconnae, An. marajoara and An. oryzalimnetes), and also support species level status for two previously detected lineages - An. albitarsis G &An. albitarsis I (designated herein). In addition, we highlight the presence of a unique mitochondrial lineage close to An. deaneorum and An. marajoara (An. albitarsis H) from Rondônia and Mato Grosso in southwestern Brazil. Further integrated studies are required to confirm the status of this lineage. DNA barcoding provides a reliable means of identifying both known and undiscovered biodiversity within the closely related taxa of the Albitarsis Group. We advocate its usage in future studies to elucidate the vector competence and respective distributions of all eight species in the Albitarsis Group and the novel mitochondrial lineage (An. albitarsis H) recovered in this study.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
DOI：
10.1590/s0074-02762010000800011
发表时间：
2010-12-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Gutiérrez, Lina A;Orrego, Lina M;Correa, Margarita M
通讯作者：
Correa, Margarita M
DOI：
10.4269/ajtmh.2007.76.1113
发表时间：
2007-06-01
影响因子：
3.3
作者：
Brochero, Helena H. L.;Li, Cong;Wilkerson, Richard C.
通讯作者：
Wilkerson, Richard C.
影响因子：
4.1
作者：
Krzywinski, Jaroslaw;Li, Cong;Morris, Marion;Conn, Jan E.;Lima, Jose B.;Povoa, Marinete M.;Wilkerson, Richard C.
通讯作者：
Wilkerson, Richard C.
影响因子：
1.9
作者：
Moreno, JE;Rubio-Palis, Y;Vaccari, E
通讯作者：
Vaccari, E