Extracellular vesicles derived from Malassezia furfur stimulate IL-6 production in keratinocytes as demonstrated in in vitro and in vivo models.
Extracellular vesicles derived from Malassezia furfur stimulate IL-6 production in keratinocytes as demonstrated in in vitro and in vivo models.
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体外和体内模型证明，源自糠秕马拉色菌的细胞外囊泡可刺激角质形成细胞中 IL-6 的产生。
DOI：
10.1016/j.jdermsci.2019.03.001
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Gao XH
中科院分区：
文献类型：
--
作者：
Zhang YJ;Han Y;Sun YZ;Jiang HH;Liu M;Qi RQ;Gao XH
背景：马拉色菌是一种定植于人体皮肤上的共生微生物，已被证明与多种炎症性皮肤病有关。先前的研究表明马拉色菌。合胞杆菌能产生胞外囊泡，但马拉色菌胞外囊泡对角质形成细胞的免疫调节作用尚未见报道。目的：探讨马拉色菌胞外囊泡的产生能力。方法：用序贯超速离心法分离培养的呋喃沙门氏菌胞外囊泡。用负染色透射电子显微镜和NTA分别测定了它们的结构和直径。使用共聚焦显微镜观察这些纳米颗粒内化到HaCaT细胞和小鼠表皮角质形成细胞中的情况。用聚合酶链式反应芯片法筛选炎性细胞因子的表达，并用定量聚合酶链式反应和酶联免疫吸附试验进行体外验证。用免疫组织化学方法检测体内细胞因子的产生。结果：我们的结果表明，呋喃支原体产生卵圆形纳米颗粒，然后可以内化到HaCaT细胞和小鼠表皮角质形成细胞中。体内外细胞外小泡刺激后IL-6的表达均显著增强，这一过程与NF-κB的激活有关。结论：呋喃支原体具有释放细胞外小泡的能力，这些囊泡可内化到角质形成细胞内，并通过参与NF-κB依赖的途径促进IL-6的产生。这些发现揭示了对马拉色菌发病机制和治疗的一些重要的新见解。
Abstract.BACKGROUND:.Malassezia is one of the commensal microorganisms colonized on human skin and has been shown to be related to several inflammatory cutaneous disorders. Previous studies indicated that Malassezia. sympodialis (M. sympodialis) can produce extracellular vesicles, however, the immunoregulatory function of Malassezia extracellular vesicles on keratinocytes has not been studied...OBJECTIVE:.To investigate the extracellular vesicular production capability of Malassezia. furfur (M. furfur) and examine their immunoregulatory effects both in vitro and in vivo...METHODS:.Extracellular vesicles derived from M. furfur were isolated by sequential ultracentrifugation procedure. Their structure and diameter were determined by negative stain TEM and NTA, respectively. Confocal microscopy was used to visualize the internalization of these nanoparticles into HaCaT cells and mice epidermal keratinocytes. The expressions of inflammatory cytokines were screened using PCR Array assay and validated in vitro by qPCR and ELISA assays. In vivo cytokine production was measured by the IHC method. The role of NF-κB in such process was evaluated in HaCaT cells by western blot assay...RESULTS:.Our results showed that M. furfur produced ovoid-shaped nanoparticles, which could be then internalized into HaCaT cells, as well as mice epidermal keratinocytes. IL-6 expression was significantly enhanced in response to extracellular vesicular stimulation both in vitro and in vivo, in which process the activation of NF-κB was involved...CONCLUSION:.M. furfur has the ability to release extracellular vesicles, which can be internalized into keratinocytes and promote the production of IL-6 with the involvement of NF-κB dependent pathway. Such findings reveal some important new insights into Malassezia pathogenesis and therapy.
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影响因子：
1.9
作者：
Park M;Do E;Jung WH
通讯作者：
Jung WH
影响因子：
3.7
作者：
Huang SH;Wu CH;Chang YC;Kwon-Chung KJ;Brown RJ;Jong A
通讯作者：
Jong A
DOI：
10.1046/j.1523-1747.2001.01321.x
发表时间：
2001-05
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Shinichi Watanabe;Rui Kano;Rui Kano;Hiroko Sato;Yuka Nakamura;Yuka Nakamura;Atsuhiko Hasegawa
通讯作者：
Shinichi Watanabe;Rui Kano;Rui Kano;Hiroko Sato;Yuka Nakamura;Yuka Nakamura;Atsuhiko Hasegawa
影响因子：
4.9
作者：
Balato, Anna;Paoletti, Iole;Donnarumma, Giovanna
通讯作者：
Donnarumma, Giovanna
DOI：
10.1073/pnas.0706756104
发表时间：
2007-11-20
影响因子：
11.1
作者：
Xu, Jun;Saunders, Charles W.;Dawson, Thomas L., Jr.
通讯作者：
Dawson, Thomas L., Jr.