Comparative genetic analysis of the 45S rDNA intergenic spacers from three Saccharum species.
Comparative genetic analysis of the 45S rDNA intergenic spacers from three Saccharum species.
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三种甘蔗属植物 45S rDNA 基因间间隔区的比较遗传分析
DOI：
10.1371/journal.pone.0183447
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Huang Y;Yu F;Li X;Luo L;Wu J;Yang Y;Deng Z;Chen R;Zhang M
45S核糖体DNA（rDNA）单元由基因间间隔区（IGS）分隔，所述基因间间隔区含有用于rRNA的转录和加工的信号。我们首次对三个原属物种的IGS区域进行了测序和分析，包括S。spontaneum，密生草S. robustum和S.在这项研究中，我们比较了三个原产种的IGS组织。这三个原始物种的IGS显示出相似的整体组织，包括rRNA基因活性所需的推定功能元件以及非转录间隔区（NTS），启动子区和外部转录间隔区（ETS）。IGS序列长度的变异性在个体、种内和种间水平上进行了评估。spontaneum，密生草S. robustum和S.药用植物。ETS有更大的相似性比NTS跨物种，但仍表现出长度的变化。在拟南芥的IGS序列中，碱基替换和亚重复序列拷贝数的变异是导致IGS序列差异的原因。我们还发现了大量的甲基化位点。此外，荧光原位杂交（FISH）共定位的IGS和pTa71探针检测到所有代表性物种的属测试。综上所述，本研究的结果提供了一个更好的洞察的结构和组织的IGS在属的植物。
The 45S ribosomal DNA (rDNA) units are separated by an intergenic spacer (IGS) containing the signals for transcription and processing of rRNAs. For the first time, we sequenced and analyzed the entire IGS region from three original species within the genus Saccharum, including S. spontaneum, S. robustum, and S. officinarum in this study. We have compared the IGS organization within three original species of the genus Saccharum. The IGS of these three original species showed similar overall organizations comprised of putative functional elements needed for rRNA gene activity as well as a non-transcribed spacer (NTS), a promoter region, and an external transcribed spacer (ETS). The variability in length of the IGS sequences was assessed at the individual, intraspecies, and interspecies levels of the genus Saccharum, including S. spontaneum, S. robustum, and S. officinarum. The ETS had greater similarity than the NTS across species, but nevertheless exhibited variation in length. Within the IGS of the Saccharum species, base substitutions and copy number variation of sub-repeat were causes of the divergence in IGS sequences. We also identified a significant number of methylation sites. Furthermore, fluorescent in situ hybridization (FISH) co-localization of IGS and pTa71 probes was detected on all representative species of the genus Saccharum tested. Taken together, the results of this study provide a better insight into the structure and organization of the IGS in the genus Saccharum.
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影响因子：
14.9
作者：
Goujon M;McWilliam H;Li W;Valentin F;Squizzato S;Paern J;Lopez R
通讯作者：
Lopez R
影响因子：
4.2
作者：
Dillon SL;Shapter FM;Henry RJ;Cordeiro G;Izquierdo L;Lee LS
通讯作者：
Lee LS
影响因子：
5.4
作者：
FUKUI, K;OHMIDO, N;KHUSH, GS
通讯作者：
KHUSH, GS
影响因子：
7
作者：
Ganley, Austen R. D.;Kobayashi, Takehiko
通讯作者：
Kobayashi, Takehiko
影响因子：
1.9
作者：
BREMER, G
通讯作者：
BREMER, G