Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts at the Early Stage and Improve Long-Term Retention in Immunocompetent Rats
Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts at the Early Stage and Improve Long-Term Retention in Immunocompetent Rats
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同种异体脂肪干细胞在早期阶段保护脂肪移植物并改善免疫活性大鼠的长期保留
DOI：
10.1007/s00266-015-0505-9
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发表时间：
2015-06
影响因子：
2.4
通讯作者：
Liu Xiaoyan
中科院分区：
文献类型：
--
作者：
Zhang Jun;Wang Yunchuan;Zhao Bin;Fan Lei;Bai Xiaozhi;Yang Longlong;Chang Peng;Hu Dahai;Liu Xiaoyan
背景同基因脂肪源性干细胞（ASCs）促进脂肪移植物的存活。但目前尚不清楚同种异体ASC是否具有类似的保护作用。在这项研究中，我们研究了同种异体ASCs在脂肪移植模型中的免疫功能正常的rats.MethodsSyngeneic和同种异体ASCs的保护作用，分别来自刘易斯（LEW）和Norway-Brown大鼠。54只LEW大鼠分为3组。每只LEW大鼠在两个椎旁皮下注射与DMEM（AFT组）、同基因ASC（SYNG组）或同种异体ASC（ALLG组）预混合的脂肪颗粒。在第7天和第14天收获脂肪移植物以检测细胞凋亡率，并对Perilipin A和CD 34进行免疫组织化学染色。在3个月时，测量脂肪移植物体积保留。结果14 d时ALLG组外周血中Perilipin A、CD 34的表达均高于AFT组（P< 0.05）; ALLG组细胞凋亡率在第7、14天均低于AFT组（P< 0.05）。3个月时，同种异体ASCs增加了脂肪移植物的体积保留（P< 0.05）。Treg细胞比例和CD 4/CD 8细胞比例在各组间无差异。ALLG和SYNG组在所有时间点的差异均无统计学意义（P> 0.05）.结论同种异体ASCs在早期阶段保护了脂肪移植物，并改善了免疫活性大鼠脂肪移植模型的长期体积保留，没有或很少有明显的免疫排斥反应。基于医学的排名是适用的。这不包括评论文章，书评，以及涉及基础科学，动物研究，尸体研究和实验研究的手稿。有关这些循证医学评级的完整描述，请参阅目录或在线作者说明 www.springer.com/00266 .
BackgroundSyngeneic adipose-derived stem cells (ASCs) promote the survival of fat grafts. But it is unclear whether allogeneic ASCs have a similar protective effect. In this study, we investigated the protective effect of allogeneic ASCs in a fat graft model of immunocompetent rats.MethodsSyngeneic and allogeneic ASCs were derived from Lewis (LEW) and Norway-Brown rats, respectively. Fifty-four LEW rats were divided into three groups. Each LEW rat was injected subcutaneously at two paravertebral spots with adipose granules premixed with DMEM (AFT group), syngeneic ASCs (SYNG group), or allogeneic ASCs (ALLG group). Fat grafts were harvested at 7 and 14 days to examine apoptosis rates and immunochemistry staining was performed for Perilipin A and CD34. At 3 months, fat graft volume retentions were measured. The proportion of regulatory T (Treg) cells and the ratio of CD4/CD8 cells in blood were analyzed at 7 days.ResultsExpression of Perilipin A and CD34 was higher in the ALLG group than the AFT group at 14 days (P< 0.05). The apoptosis rate in the ALLG group decreased in comparison with the AFT group at 7 and 14 days (P< 0.05). At 3 months, allogeneic ASCs increased fat graft volume retentions (P< 0.05). No difference was found in the proportion of Treg cells and CD4/CD8 cells ratio between groups. There were no statistically significant difference between ALLG and SYNG groups at all time points (P> 0.05).ConclusionsAllogeneic ASCs protected fat grafts at the early stage and improved long-term volume retention in the fat graft model of immunocompetent rats with no or little obvious immune rejection.No Level AssignedThis journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
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影响因子：
7.2
作者：
Piccinno, Maria Serena;Veronesi, Elena;Loschi, Pietro;Pignatti, Marco;Murgia, Alba;Grisendi, Giulia;Castelli, Ilaria;Bernabei, Daniela;Candini, Olivia;Conte, Pierfranco;Paolucci, Paolo;Horwitz, Edwin M.;De Santis, Giorgio;Iughetti, Lorenzo;Dominici, Massimo
通讯作者：
Dominici, Massimo
影响因子：
7.4
作者：
Choudhery MS;Badowski M;Muise A;Pierce J;Harris DT
通讯作者：
Harris DT
影响因子：
7.5
作者：
Gutiérrez-Fernández M;Rodríguez-Frutos B;Ramos-Cejudo J;Teresa Vallejo-Cremades M;Fuentes B;Cerdán S;Díez-Tejedor E
通讯作者：
Díez-Tejedor E
影响因子：
2.9
作者：
Cianfarani, Francesca;Toietta, Gabriele;Odorisio, Teresa
通讯作者：
Odorisio, Teresa
影响因子：
3.3
作者：
Y. Kubota;K. Kishi;H. Satoh;Takara Tanaka;H. Nakajima;T. Nakajima
通讯作者：
Y. Kubota;K. Kishi;H. Satoh;Takara Tanaka;H. Nakajima;T. Nakajima