Accuracy of regenerating motor neurons: influence of diffusion in denervated nerve.
Accuracy of regenerating motor neurons: influence of diffusion in denervated nerve.
复制标题
DOI：
10.1016/j.neuroscience.2014.05.016
复制
发表时间：
2014-07-25
期刊：
影响因子：
3.3
通讯作者：
Robinson, G. A.
中科院分区：
文献类型：
--
作者：
Madison, R. D.;Robinson, G. A.
外周神经损伤后，去神经支配的远端神经段发生显著变化，包括血神经屏障丧失、雪旺细胞增殖、巨噬细胞入侵以及许多细胞因子和神经营养因子的产生。这些变化的总体结果是，失神经支配的神经成为近端神经段再生轴突的一个允许的甚至是首选的目标。在再生期间，原始末端器官靶点（如肌肉）在这种现象中可能发挥的作用在很大程度上尚未被探索。我们使用大鼠股神经作为体内模型来开始解决这个问题。我们还研究了中断与肌肉的交流对运动神经元再生准确性的影响，通过运动神经元正确投射到原始末梢神经分支的能力来判断运动神经元再生的准确性。我们的研究结果表明，运动神经元再生的准确性取决于失神经节段与肌肉保持不间断的连续性。我们假设，肌肉对去神经支配神经的影响可能是通过扩散驱动的生物分子运动或在大鼠被切断的轴突中持续数天的活跃轴突运输，因此我们设计了实验来分离这两种可能性。我们的数据表明，破坏失神经中正在进行的扩散驱动运动显著降低了运动神经元再生的准确性。
Following injury to a peripheral nerve the denervated distal nerve segment undergoes remarkable changes including loss of the blood-nerve barrier, Schwann cell proliferation, macrophage invasion, and the production of many cytokines and neurotrophic factors. The aggregate consequence of such changes is that the denervated nerve becomes a permissive and even preferred target for regenerating axons from the proximal nerve segment. The possible role that an original end-organ target (e.g. muscle) may play in this phenomenon during the regeneration period is largely unexplored. We used the rat femoral nerve as an in vivo model to begin to address this question. We also examined the effects of disrupting communication with muscle in terms of the accuracy of regenerating motor neurons as judged by their ability to correctly project to their original terminal nerve branch. Our results demonstrate that the accuracy of regenerating motor neurons is dependent upon the denervated nerve segment remaining in uninterrupted continuity with muscle. We hypothesized that this influence of muscle on the denervated nerve might be via diffusion driven movement of biomolecules or the active axonal transport that continues in severed axons for several days in the rat, so we devised experiments to separate these two possibilities. Our data show that disrupting ongoing diffusion driven movement in a denervated nerve significantly reduces the accuracy of regenerating motor neurons.
登录
查看更多内容
影响因子：
2.5
作者：
English, Arthur W.;Chen, Yi;Chen, Xiang Yang
通讯作者：
Chen, Xiang Yang
影响因子：
1.5
作者：
DELLON, AL;MACKINNON, SE
通讯作者：
MACKINNON, SE
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
影响因子：
16.2
作者：
Brückner, K;Labrador, JP;Klein, R
通讯作者：
Klein, R
影响因子：
4.7
作者：
HASSIG, R;TAVITIAN, B;DIGIAMBERARDINO, L
通讯作者：
DIGIAMBERARDINO, L