Transcriptional control of Rohon-Beard sensory neuron development at the neural plate border.
Transcriptional control of Rohon-Beard sensory neuron development at the neural plate border.
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DOI：
10.1002/dvdy.21915
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发表时间：
2009-04
影响因子：
2.5
通讯作者：
Artinger, Kristin Bruk
中科院分区：
文献类型：
--
作者：
Rossi, Christy Cortez;Kaji, Takao;Artinger, Kristin Bruk
Rohon-Beard（RB）机械感觉神经元是最早发育的感觉神经元之一，它们接受命运的过程尚未完全了解。RB形成于神经板边界（NPB），神经和表皮外胚层之间的交界处，并需要转录因子prdm 1a。在这里，我们表明，RB分化前，prdm 1a广泛重叠的表皮标记dlx 3b，但显示与神经外胚层标记sox 3和sox 19 a的重叠很少。分析表明，大多数RB在斑马鱼原肠胚形成期间出生，这表明在此期间，RB变得特定。prdm 1a和neurogenin 1突变体和dlx 3b/dlx 4 b吗啉注射胚胎的表达分析表明，prdm 1a是上游的dlx 3b，dlx 4 b，和neurogenin 1在NPB。neurogenin 1或dlx 3b/dlx 4 b的mRNA可以挽救prdm 1a中RB的缺乏。基于这些数据，我们提出了一个初步的基因调控网络RB的发展。
Rohon-Beard (RB) mechanosensory neurons are among the first sensory neurons to develop, and the process by which they adopt their fate is not completely understood. RBs form at the neural plate border (NPB), the junction between neural and epidermal ectoderm, and require the transcription factor prdm1a. Here, we show that prior to RB differentiation, prdm1a overlaps extensively with the epidermal marker dlx3b but shows little overlap with the neuroectodermal markers sox3 and sox19a. Birthdating analysis reveals that the majority of RBs are born during gastrulation in zebrafish, suggesting that it is during this period that RBs become specified. Expression analysis in prdm1a and neurogenin1 mutant and dlx3b/dlx4b morpholino-injected embryos suggests that prdm1a is upstream of dlx3b, dlx4b, and neurogenin1 at the NPB. mRNA for neurogenin1 or dlx3b/dlx4b can rescue the lack of RBs in prdm1a. Based on these data we suggest a preliminary gene regulatory network for RBs development.
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影响因子：
3.8
作者：
Roberts, A
通讯作者：
Roberts, A
影响因子：
2.7
作者：
Rossi, Christy Cortez;Hernandez-Lagunas, Laura;Artinger, Kristin Bruk
通讯作者：
Artinger, Kristin Bruk
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
影响因子：
4.6
作者：
Pineda, Ricardo H.;Svoboda, Kurt R.;Ribera, Angeles B.
通讯作者：
Ribera, Angeles B.
影响因子：
2.5
作者：
Okuda, Y;Yoda, H;Kamachi, Y
通讯作者：
Kamachi, Y