Cell therapy using articular chondrocytes overexpressing BMP-7 or BMP-10 in a rabbit disc organ culture model.
Cell therapy using articular chondrocytes overexpressing BMP-7 or BMP-10 in a rabbit disc organ culture model.
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在兔椎间盘器官培养模型中使用过表达 BMP-7 或 BMP-10 的关节软骨细胞进行细胞治疗。
DOI：
10.1097/brs.0b013e31816b1f38
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发表时间：
2008
期刊：
影响因子：
3
通讯作者：
Anderson,DGreg
中科院分区：
文献类型：
--
作者：
Zhang,Yejia;Phillips,FrankM;Thonar,EugeneJ-MA;Oegema,Theodore;An,HowardS;Roman-Blas,JorgeA;He,Tong-Chuan;Anderson,DGreg
研究设计：将过表达人类生长因子的兔膝关节软骨细胞注射到培养的椎间盘外植体中。注射细胞的存活率和细胞外基质的积累进行了assessed.Objective.To确定基于细胞的基因传递方法修复椎间盘的实用性。背景资料摘要：与症状性椎间盘退变相关的背痛是一种常见的临床疾病。生长因子刺激椎间盘细胞代谢，但体内递送的理想方法尚未建立。方法：新西兰白色兔关节软骨细胞用表达人骨形态发生蛋白-7和绿色荧光蛋白（GFP）的腺病毒转导，将人骨形态发生蛋白-10（AdhBMP-7）、人骨形态发生蛋白-10和GFP（AdBMP-10）或单独的GFP（AdGFP，作为对照）注射到整个椎间盘外植体中。将椎间盘在培养物中保持1至2个月。在培养期结束时，通过荧光显微镜和细胞外基质的积累进行了评估与生化methods.Results.Chondrocytes实现了长期生存的培养光盘外植体细胞存活进行了评估。软骨细胞/BMP-7处理的光盘表现出髓核内的蛋白多糖含量增加50%，与对照组（软骨细胞/GFP）相比，而注射软骨细胞/BMP-10的光盘未能表现出显着增加蛋白多糖accumulation.Conclusion.Our的研究表明，转导的关节软骨细胞的生存能力，并促进蛋白多糖积累时，移植到椎间盘。这些数据支持基于细胞的基因治疗方法用于椎间盘修复的潜力。在动物模型中使用这种方法的进一步研究表明，这是实现人类椎间盘修复的一个步骤。
Study Design.Rabbit knee articular chondrocytes overexpressing human growth factors were injected into cultured intervertebral disc explants. Survival of the injected cells and accumulation of extracellular matrix were assessed.Objective.To define the utility of cell-based gene delivery approach for repair of the intervertebral disc.Summary of Background Data.Back pain associated with symptomatic disc degeneration is a common clinical condition. Growth factors stimulate disc cell metabolism, but the ideal method for in vivo delivery has not been established. Cells as a vehicle for delivering growth factors to the disc offer potential advantages, including prolonged production of the growth factor within the disc and vital cells to participate in the repair process.Methods.New Zealand white rabbit articular chondrocytes transduced with adenovirus expressing human bone morphogenetic protein-7 and green fluorescence protein (GFP)(AdhBMP-7), human bone morphogenetic protein-10 and GFP (AdBMP-10), or GFP alone (AdGFP, as a control) were injected into whole disc explants. Discs were maintained in culture for 1 to 2 months. At the conclusion of the culture periods, cell survival was assessed by fluorescence microscopy and extracellular matrix accumulation was assessed with biochemical methods.Results.Chondrocytes achieved long-term survival in the cultured disc explants. The discs treated with chondrocytes/BMP-7 demonstrated a 50% increase in proteoglycan content within the nucleus pulposus compared to control (chondrocytes/GFP), while discs injected with chondrocytes/BMP-10 failed to show a significant increase in proteoglycan accumulation.Conclusion.Our study demonstrates the ability of transduced articular chondrocytes to survive and promote proteoglycan accumulation when transplanted into the intervertebral disc. These data support the potential of a cell-based gene therapy approach for disc repair. Further studies using this approach in animal models are indicated as a step towards achieving disc repair in humans.
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DOI：
--
发表时间：
1982
期刊：
The Lancet
影响因子：
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作者：
D. Wathes;B. Pickering;R. W. Swann;D. G. Porter;M. Hull;J. Drife
通讯作者：
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影响因子：
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作者：
Hoyer,PB;Fitz,TA;Niswender,GD
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影响因子：
4.8
作者：
C. Channing
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C. Channing
月经周期期间从恒河猴分离的黄体细胞产生雌激素：与自发黄体溶解的相关性。
影响因子：
4.8
作者：
R. Stouffer;Laura A. Bennett;G. D. Hodgen
通讯作者：
G. D. Hodgen
恒河猴黄体细胞在体外产生促性腺激素敏感的黄体酮：月经周期期间黄体年龄的函数。
影响因子：
4.8
作者：
R. Stouffer;W. E. Nixon;B. Gulyas;G. Hodgen
通讯作者：
G. Hodgen