Blue light inhibits transforming growth factor‐β1‐induced myofibroblast differentiation of human dermal fibroblasts
Blue light inhibits transforming growth factor‐β1‐induced myofibroblast differentiation of human dermal fibroblasts
复制标题
蓝光抑制转化生长因子β1诱导的人真皮成纤维细胞分化
DOI：
10.1111/exd.12353
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发表时间：
2014
影响因子：
3.6
通讯作者：
C. Opländer
中科院分区：
文献类型：
--
作者：
Taflinski L;E. Demir;J. Kauczok;P. C. Fuchs;M. Born;C. V. Suschek;C. Opländer
转化生长因子-β-1是成纤维细胞-肌成纤维细胞表型转化的主要促进剂，同时伴随着肌动蛋白-平滑肌肌动蛋白(α-α-SMA)的表达和掺入。这种分化在正常的伤口愈合和愈合过程中至关重要；然而，肌成纤维细胞被认为是纤维化的主要效应细胞类型，例如在硬皮病和增生性瘢痕形成中。由于蓝光在几种细胞类型中具有抑制繁殖和毒性的作用，我们研究了使用发光二极管阵列(420 Nm)的蓝光照射是否能够影响人真皮成纤维细胞(HDF)的增殖和分化。我们发现，无毒剂量的重复照射显著抑制转化生长因子-β-1诱导的人成纤维细胞向肌成纤维细胞的分化，α-SMA免疫细胞化学和Western blotting显示。此外，使用的剂量减少了通过丽珠灵和胶原凝胶收缩试验测量的肌纤维细胞的增殖和收缩能力。可以证明，蓝光通过产生单线态氧引起的氧化应激来介导细胞毒性。我们推测，无毒剂量的辐射诱导低水平的氧化应激和耗能的细胞反应，这两者都可能影响增殖停止和干扰肌成纤维细胞的分化。因此，蓝光靶向肌成纤维细胞的分化、增殖和活性可能是预防或减少病理性纤维化的有效策略。
Transforming growth factor‐β1 (TGF‐β1) is the major promoter of phenotypic shift between fibroblasts and myofibroblasts accompanied by the expression and incorporation ofα‐smooth muscle actin (α‐SMA). This differentiation is crucial during normal wound healing and wound closure; however, myofibroblasts are considered as the main effecter cell type in fibrosis, for example in scleroderma and hypertrophic scarring. As blue light has exerted antiprolific and toxic effects in several cell types, we investigated whether blue light irradiations with a light‐emitting diode array (420 nm) were able to affect proliferation and differentiation of human dermal fibroblasts (HDF). We found that repeated irradiation with non‐toxic doses significantly inhibits TGF‐β1‐induced differentiation of HDF into myofibroblasts shown byα‐SMA immunocytochemistry and Western blotting. Additionally, used doses reduced proliferation and myofibroblast contractibility measured by resazurin and collagen gel contraction assays. It could be demonstrated that blue light mediates cell toxicity by oxidative stress due to the generation of singlet oxygen. We postulate that irradiations at non‐toxic doses induce low‐level oxidative stress and energy‐consuming cellular responses, which both may effect proliferation stop and interfere with myofibroblast differentiation. Thus, targeting differentiation, proliferation and activity of myofibroblasts by blue light may represent a useful strategy to prevent or reduce pathological fibrotic conditions.
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影响因子：
3.3
作者：
MATSUNAGA, T;HIEDA, K;NIKAIDO, O
通讯作者：
NIKAIDO, O
DOI：
10.1016/s1357-2725(01)00041-3
发表时间：
2001-07-01
影响因子：
4
作者：
Roy, SG;Nozaki, Y;Phan, SH
通讯作者：
Phan, SH
影响因子：
1.5
作者：
Elaine Shnitkind;E. Yaping;S. Geen;A. Shalita;W. Lee
通讯作者：
W. Lee
影响因子：
4
作者：
A. Tingström;C. Heldin;K. Rubin
通讯作者：
A. Tingström;C. Heldin;K. Rubin
影响因子：
5.7
作者：
Ohara, M;Kawashima, Y;Watanabe, H
通讯作者：
Watanabe, H