Development of chemosensitivity in neurons from the nucleus tractus solitarii (NTS) of neonatal rats.
Development of chemosensitivity in neurons from the nucleus tractus solitarii (NTS) of neonatal rats.
复制标题
新生大鼠的神经元（NTS）神经元中化学敏度的发展。
DOI：
10.1016/j.resp.2008.11.005
复制
发表时间：
2009-03-31
影响因子：
2.3
通讯作者：
Putnam, Robert W.
中科院分区：
文献类型：
--
作者：
Conrad, Susan C.;Nichols, Nicole L.;Ritucci, Nick A.;Dean, Jay B.;Putnam, Robert W.
我们研究了新生大鼠孤束核(NTS)神经元对化疗药物敏感性的发展。我们测定了高碳酸血症(15%CO2)激活神经元的百分比，并通过计算化疗敏感性指数(CI)来评估反应的大小。不同年龄段新生大鼠NTS神经元被高碳酸血症抑制(9%)或激活(44.8%)的神经元百分率及激活反应的大小(CI 164±4.9%)无明显差异。为了评估这些神经元的内在化学敏感性，我们使用了化学突触阻滞剂和缝隙连接阻滞剂Carbenoxolone。化学突触阻滞液略微降低基础放电率，但不影响任何新生儿年龄对高碳酸血症反应的NTS神经元的百分比。然而，在P10岁的新生儿中，化学突触阻断介质增加了CI，但在大龄新生儿中没有。在任何年龄的新生大鼠中，Carbenoxolone并不显著改变高碳酸血症所激活的NTS神经元数量。综上所述，新生大鼠NTS神经元的反应似乎是内在的，在整个早期发育过程中基本没有变化。在年轻的新生儿(<；P10)中，化学突触输入降低了对高碳酸血症的放电率反应的幅度，但除此之外，化学突触输入和缝隙连接都不会显著改变NTS神经元对高二氧化碳反应的百分比或反应的幅度。
We studied the development of chemosensitivity during the neonatal period in rat Nucleus tractus solitarii (NTS) neurons. We determined the percentage of neurons activated by hypercapnia (15% CO2) and assessed the magnitude of the response by calculating the chemosensitivity index (CI). There were no differences in the percentage of neurons that were inhibited (9%) or activated (44.8%) by hypercapnia or in the magnitude of the activated response (CI 164±4.9%) in NTS neurons from neonatal rats of all ages. To assess the degree of intrinsic chemosensitivity in these neurons we used chemical synaptic block medium and the gap junction blocker carbenoxolone. Chemical synaptic block medium slightly decreased basal firing rate but did not affect the percentage of NTS neurons that responded to hypercapnia at any neonatal age. However, in neonates aged <P10, but not in older neonates, chemical synaptic block medium increased CI. Carbenoxolone did not significantly alter the number of NTS neurons activated by hypercapnia in neonatal rats of any age. In summary, the response of NTS neurons from neonatal rats appears to be intrinsic and largely unchanged throughout early development. In young neonates (<P10) chemical synaptic input reduces the magnitude of the firing rate response to hypercapnia, but otherwise neither chemical synaptic input nor gap junctions significantly alter the percentage of NTS neurons that respond to hypercapnia or the magnitude of that response.
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DOI：
10.1016/s0034-5687(01)00284-5
发表时间：
2001-12-01
期刊：
RESPIRATION PHYSIOLOGY
影响因子：
--
作者：
Dean, JB;Kinkade, EA;Putnam, RW
通讯作者：
Putnam, RW
影响因子：
3.3
作者：
Nattie, EE;Li, AH
通讯作者：
Li, AH
影响因子：
3.3
作者：
Mulkey, DK;Henderson, RA;Dean, JB
通讯作者：
Dean, JB
影响因子：
2.3
作者：
Parisian, K;Wages, P;Erlichman, JS
通讯作者：
Erlichman, JS
影响因子：
5.5
作者：
Oyamada, Y;Ballantyne, D;Scheid, P
通讯作者：
Scheid, P